# Mitigation of the Negative Effect of Drought and Herbicide Treatment on Growth, Yield, and Stress Markers in Bread Wheat as a Result of the Use of the Plant Growth Regulator Azolen®

## TL;DR

Azolen® helps wheat plants grow better under drought and herbicide stress by improving their hormonal balance and protecting their roots.

## Contribution

Azolen® is shown to mitigate herbicide and drought stress in wheat by altering plant hormone levels and improving root function.

## Key findings

- Azolen® reduced abscisic acid and normalized auxin and cytokinin levels in wheat under stress.
- Azolen® treatment prevented herbicide-induced root growth inhibition and improved water absorption.
- Wheat yield increased 1.6 times with Azolen® under drought and herbicide stress.

## Abstract

Most chemical pesticides, in addition to their main functions (protection against diseases, weeds, and pests), also have a noticeable inhibitory effect on target crops. In a laboratory experiment and two-year field experiments (Russia, Trans-Urals), a study was made of the effect of the biopreparation Azolen® (Azotobacter vinelandii IB-4) on plants of the Ekada 113 wheat variety under conditions of drought and stress caused by the exposure to the herbicide Chistalan (2.4-D and dicamba). The biopreparation and the herbicide were used separately and together on wheat during the tillering phase. Treatment with the biological preparation under stressful conditions had a significant effect on the hormonal balance of plants (a decrease in the amount of abscisic acid and a normalization of the balance of indolyl-3-acetic acid and cytokinins in shoots and roots of plants was noted), while the osmoprotective, antioxidant, and photosynthetic systems of plants were activated. In drought conditions, the treatment of plants with biological preparation prevented the inhibition of root growth caused by the use of the herbicide. This, in turn, improved the absorption of water by plants and ensured an increase in wheat yield (1.6 times). The results obtained give reason to believe that microbiological preparations can be used as antidotes that weaken the phytotoxic effect of herbicidal treatments, including in drought conditions.