# Optimization and Spectrum–Effect Analysis of Ultrasonically Extracted Antioxidant Flavonoids from Persicae Ramulus

## TL;DR

This study optimized the extraction of antioxidant flavonoids from Persicae Ramulus and identified key compounds responsible for their antioxidant effects.

## Contribution

The study introduces a spectrum–effect analysis to identify antioxidant components in Persicae Ramulus using optimized ultrasonic extraction.

## Key findings

- Optimal flavonoid extraction was achieved using 53% ethanol, a 1:26 solid–liquid ratio, and 60 minutes of ultrasonic extraction.
- Four characteristic peaks (peaks 4, 12, 21, and 24) were identified as responsible for the antioxidant activity of PR.
- The similarity of UHPLC fingerprints among 28 batches of PR ranged from 0.801 to 0.949.

## Abstract

The objectives of this study were to optimize the ultrasonic-assisted flavonoid extraction process from PR and to establish fingerprints in order to analyze the spectrum–effect relationship of antioxidant activity. The ultrasonic-assisted flavonoid extraction process from PR was optimized using RSM, and the fingerprints of twenty-eight batches of flavonoids from PR were established using UHPLC. Meanwhile, the in vitro antioxidant activity of PR was evaluated in DPPH and ABTS free radical-scavenging experiments. Then, the peaks of the effective antioxidant components were screened using the spectrum–effect relationships. The results show that the optimal extraction yield of flavonoids from PR was 3.24 ± 0.01 mg/g when using 53% ethanol, a 1:26 (g/mL) solid–liquid ratio, and 60 min of ultrasonic extraction. Additionally, the clearance of two antioxidant indices by the flavonoids extracted from PR had different degrees of correlation and showed concentration dependence. Simultaneously, the similarity of the UHPLC fingerprints of twenty-eight batches of PR samples ranged from 0.801 to 0.949, and four characteristic peaks, namely peaks 4, 12, 21, and 24, were screened as the peaks of the components responsible for the antioxidant effect of PR using a GRA, a Pearson correlation analysis, and a PLS-DA. In this study, characteristic peaks of the antioxidant effects of PR were screened in an investigation of the spectrum–effect relationship to provide a scientific basis for the study of pharmacodynamic substances and the elucidation of the mechanism of action of the antioxidant effect of PR.