# Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide Studies

## TL;DR

Researchers developed a new culture medium to study how lanthanides interact with microbes, enabling better solubility and microbial growth.

## Contribution

A novel minimal culture medium was developed to solubilize lanthanides and support microbial growth for studying their biological interactions.

## Key findings

- The new medium solubilizes Ln3+ up to 100 times more than other media.
- It supports growth of 63 bacteria and 5 fungi, including a Bacillus strain that tolerates high lanthanide concentrations.
- The medium allows for superior microbial growth and biomass compared to existing minimal media.

## Abstract

Rare Earth Elements (REE), also known as Lanthanides (Ln3+), are a group of 17 elements showing peculiar physical and chemical properties. Unlike technological applications, very little is known about the physiological role and toxicity of Ln3+ on biological systems, in particular on microorganisms (e.g., bacteria), which represent the most abundant domains on our planet. Up to now, very limited studies have been conducted due to Ln3+ precipitation with some anions commonly present in the culture media. Therefore, the development of a minimal medium is essential to allow the study of Ln3+-microbial interactions, limiting considerably the precipitation of insoluble salts. In this regard, a new minimal culture medium capable of solubilizing large amounts of Ln3+ and allowing the growth of different microbial taxa was successfully developed. Assays have shown that the medium is capable of solubilizing Ln3+ up to 100 times more than other common culture media and allowing the growth of 63 bacteria and 5 fungi. The kinetic growth of one yeast and one Gram-positive bacterium has been defined, proving to support superior growth and biomass compared to other commonly used minimal media. Moreover, the sensitivity and uptake/absorption of a Bacillus stratosphericus strain were tested, highlighting its capability to tolerate concentrations up to 10 mM of either Cerium, Gadolinium or Lanthanum and accumulate different quantities of the three.