Could Flow Cytometry Provide New Prognostic Markers in Colorectal Cancer?

TL;DR

This study explores whether flow cytometry can identify new markers to predict survival and treatment response in colorectal cancer patients.

Contribution

The study identifies DNA index and tumor index as potential predictive biomarkers for treatment response and survival in resectable colorectal cancer.

Findings

Diploid tumors showed better median survival compared to aneuploid tumors.

DNA index and tumor index were significantly correlated with response to neoadjuvant treatment.

Higher tumor index was associated with worse survival rates.

Abstract

Background/Objectives: Colorectal cancer (CRC) is still accompanied by significant mortality, which poses the necessity of novel markers to predict treatment success and patient survival. This study aims to evaluate the prognostic and survival impact of flowytometry (FC) in CRC patients. Methods: In this prospective study, 106 surgically resectable CRC patients were included. Tissue specimens from tumor and normal mucosa were collected and analyzed by FC. DNA and tumor index were calculated. In a subgroup of 46 patients, the CD26 expression on tumor cells was estimated. These parameters were compared with patients’ tumor characteristics as stage, histology data, responsiveness to treatment, metastasis/recurrence, and, finally, patients’ survival to identify possible new biomarkers. Results: The overall survival and the disease-specific survival in our study group was 76% and 72%, respectively, during the 7-year follow up period. Diploid tumors had better median survival than the aneuploid ones. The DNA index had significant correlation to the tumor index and response to neoadjuvant treatment. Similarly, the tumor index was also significantly related to the response to neoadjuvant treatment. Patients with a higher tumor index had worst survival rates. Surprisingly, CD26 levels were not associated with any of the parameters examined and were negatively related to tumor stage and differentiation. Conclusions: FC is a rapid and reliable method of cell analysis. In CRC, it has been used for prognostic and diagnostic purposes. In this study, we have shown that DNA and tumor index could become predictive biomarkers of tumor response to neoadjuvant treatment and survival of resectable CRC patients.

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