Characterization of PEBP-like Genes and Function of Capebp1 and Capebp5 in Fruiting Body Regeneration in Cyclocybe aegerita

TL;DR

This study explores the role of PEBP-like genes in fruiting body regeneration in the fungus Cyclocybe aegerita, finding that Capebp1 and Capebp5 are crucial for this process.

Contribution

The study identifies and characterizes four new Capebp genes in C. aegerita and demonstrates their functional roles in fruiting body regeneration.

Findings

Capebp1 and Capebp5 overexpression induces lamella or fruiting body regeneration in Cyclocybe aegerita.

Knockdown of Capebp1 or Capebp5 accelerates fruiting body aging.

Five Capebp genes were identified in C. aegerita, with low sequence similarity among their protein products.

Abstract

Phosphatidylethanolamine-binding proteins (PEBPs) play a crucial role in the growth and development of various organisms. Due to the low sequence similarity compared to plants, humans, and animals, the study of pebp genes in fungi has not received significant attention. The redifferentiation of fruiting bodies is exceedingly rare in fungal development. Hitherto, only a few studies have identified the Capebp2 gene as being associated with this phenomenon in Cyclocybe aegerita. Thus, exploring the role of pebp genes in fruiting body development is imperative. In the present study, four Capebp genes (Capebp1, Capebp3, Capebp4, and Capebp5) were cloned from the AC0007 strain of C. aegerita based on genome sequencing and gene prediction. The findings indicate that the pebp family, in C. aegerita, comprises a total of five genes. Moreover, the sequence similarity was low across the five CAPEBP protein sequences in C. aegerita, and only a few conserved sequences, such as HRY and RHF, were identical. Expression analyses revealed that, similarly to Capebp2, the four Capebp genes exhibit significantly higher expression levels in the fruiting bodies than in the mycelium. Furthermore, overexpressed and RNA interference Capebp1 or Capebp5 transformants were analyzed. The results demonstrate that overexpression of Capebp1 or Capebp5 could induce the regeneration of the lamella or fruiting body, whereas the knockdown of Capebp1 or Capebp5 could lead to the accelerated aging of fruiting bodies. These findings highlight a significant role of Capebp genes in the generation of C. aegerita fruiting bodies and provide a foundation for further exploration into their involvement in basidiomycete growth and development.

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