# Evaluation of the LightCycler® PRO Instrument as a Platform for Rhesus D Typing

## TL;DR

This study evaluates a new real-time PCR platform for accurate Rhesus D blood typing, especially in cases where traditional methods are unreliable.

## Contribution

The study introduces the LightCycler® PRO as a reliable platform for Rhesus D typing with improved data integration.

## Key findings

- The LightCycler® PRO demonstrated remarkable accuracy in Rhesus D typing.
- The platform offers improved data interpretation and integration via the HL7 interface.
- Combining real-time PCR and Sanger sequencing ensures comprehensive RHD typing.

## Abstract

Rapid and reliable Rhesus D typing is crucial for blood donation centers. In instances of massive blood transfusion or reduced antigen expression, DNA-based phenotype prediction becomes mandatory. Our molecular RHD typing approach involves an initial real-time PCR for the most common aberrant RHD types in our region, RHD*01W.1 (weak D type 1), RHD*01W.2 (weak D type 2), RHD*01W.3 (weak D type 3), and RHD*07.01 (DVII). For comprehensive coverage, Sanger sequencing of RHD coding regions is performed in the case of PCR target-negative results. We evaluated the specificity and accuracy of these methods using the recently launched LightCycler® PRO real-time platform. All findings demonstrated remarkable accuracy. Notably, the LightCycler® PRO instrument offers a distinct advantage in data interpretation and integration via the HL7 interface. This study underlines the importance of including advanced molecular techniques in blood typing protocols, especially in scenarios where conventional serological methods may be insufficient.