# In vitro culture of mechanically isolated murine primary follicles in the presence of human platelet lysate PLTMax

## TL;DR

The study compares the effects of human serum albumin and human platelet lysate on the growth and viability of cultured mouse ovarian follicles.

## Contribution

The study introduces a new in vitro culture system for murine follicles using HSA and PLTMax.

## Key findings

- HSA resulted in the highest follicular growth (3.4 fold) compared to PLTMax.
- Cellular vitality remained high (70-80%) across all groups.
- PLTMax did not enhance follicular development but preserved cell vitality.

## Abstract

To develop a system for the culture of murine preantral ovarian follicles
using Human Serum Albumin (HSA) and Human Platelet Lysate (PLTMax).

Mechanically isolated preantral follicles (N=146) were obtained from Swiss
mice and cultured in DMEM:F12 medium for ten days in a 96-well plate with
conical bottom. The medium was supplemented with penicillin, streptomycin,
and equine chorionic gonadotropin. Additional proteins were tested in 4 test
groups: G1: human serum albumin (HSA), G2: human platelet lysate (PLTM), and
G3 and G4: HSA + PLTMax at lower and higher concentrations, respectively.
Cellular vitality and oocyte morphology were evaluated on day 11 of
culture.

The highest follicular growth (3.4 fold) was achieved in HSA (G1), while a
significantly lower (1.8 fold) growth was achieved in the presence of PLTM
(G2, G4) and even further reduced (1.2 fold) when HSA and PLTM were combined
(G3). Cellular vitality was close to 70-80% among the four groups, and the
highest number of intact oocytes were found in G1.

PLTM did not improve follicular development and oocyte maturation compared to
HSA but preserved cell vitality.