# The prooxidant–antioxidant balance in diagnosis and developmental prognosis of premature neonates with asphyxia

## TL;DR

This study shows that measuring the prooxidant-antioxidant balance (PAB) can help predict the prognosis of premature babies who experience asphyxia at birth.

## Contribution

The study introduces PAB as a diagnostic marker for predicting outcomes in premature neonates with asphyxia.

## Key findings

- PAB levels were significantly higher in neonates with asphyxia compared to those without.
- PAB >25 HK predicted asphyxia prognosis with 83.3% sensitivity and 81% specificity.
- Newborns with asphyxia and abnormal outcomes had the highest PAB levels (40.00 ± 60.84 HK).

## Abstract

The antioxidant system in a preterm neonate is premature. The imbalance between the prooxidant and antioxidant systems can make these neonates prone to oxidative stress. Birth asphyxia is one of the factors that can disturb this balance.

We studied the prooxidant–antioxidant balance (PAB) in the diagnosis and developmental prognosis of preterm neonates with asphyxia.

This cohort study has been conducted between 2016 and 2022 with 2 years follow-up on 183 premature neonates admitted to Ghaem Hospital Mashhad, by using a convenience sampling method. The data-collection tool and the researcher-made checklist included the mothers' and the neonate's information, and the third segment included laboratory information. PAB was studied by using standard solutions and the Enzyme immunoassays (ELISA) method. After discharging the newborns from the hospital, they were under follow-up at 6 months, 12 months, 18 months, and 24 months, by using the Denver II test. PAB was compared among newborns with asphyxia, those without asphyxia, and also newborns with normal and abnormal outcomes in both groups.

The mean ± standard deviation of the PAB factor reported is as follows: in newborns without asphyxia (21.00 ± 18.14 HK), those with asphyxia (31.00 ± 45.42 HK), in newborns with asphyxia having abnormal outcomes (40.00 ± 60.84 HK), and those having normal outcomes (21.00 ± 18.67 HK) (P ≤ 0.05). PAB results >25 HK have been used for the diagnosis of asphyxia prognosis in newborns, with 83.3% sensitivity and 81% specificity.

The PAB index showed a significant increase after asphyxia. It can be used as a diagnostic marker for the prognosis of premature newborns with asphyxia. Thus, diagnosis and prognosis of asphyxia in premature newborns can be predicted by using the PAB index.