# Both Splice Variants of Zebrafish Tmem11 Localize to the Outer Membrane of Mitochondria

## TL;DR

This study shows that both splice variants of zebrafish Tmem11 are located in the outer mitochondrial membrane, similar to findings in mammals and fruit flies.

## Contribution

The study identifies and characterizes two zebrafish Tmem11 splice variants and their mitochondrial localization.

## Key findings

- Two splice variants of zebrafish tmem11 are expressed during early development.
- Both Tmem11 variants localize to the mitochondria in cell culture.
- Tmem11 is embedded in the outer mitochondrial membrane, as shown by biochemical fractionation.

## Abstract

In mammalian and
Drosophila
systems, Transmembrane protein 11 (TMEM11) regulates mitochondrial morphology, mitophagy, and mitochondrial function. Here, we begin to expand these studies to the zebrafish model system. We identified two splice variants of
tmem11
, which are both expressed during early development. In addition, we determined that both zebrafish Tmem11 proteins localize to the mitochondria using fluorescent tags and expression in cell culture. Consistent with recent data, biochemical fractionation indicates that Tmem11 is embedded in the outer membrane of mitochondria. Overall, these studies will provide new insights into the complex protein network that mediates mitochondrial physiology in the zebrafish.