# Understanding the sources, function, and irreplaceable role of cobamides in organohalide-respiring bacteria

## TL;DR

This review explains how cobamides are crucial for bacteria that break down harmful halogenated pollutants and highlights their role in bioremediation.

## Contribution

The paper systematically summarizes the irreplaceable role of cobamides in organohalide respiration and their salvage pathways in bacteria.

## Key findings

- Cobamides are essential cofactors for reductive dehalogenases in organohalide-respiring bacteria.
- Some bacteria cannot synthesize cobamides and rely on environmental sources or co-culture with cobamide producers.
- Understanding cobamide function can improve bioremediation strategies for halogenated pollutants.

## Abstract

Halogenated organic compounds are persistent pollutants that pose a serious threat to human health and the safety of ecosystems. Cobamides are essential cofactors for reductive dehalogenases (RDase) in organohalide-respiring bacteria (OHRB), which catalyze the dehalogenation process. This review systematically summarizes the impact of cobamides on organohalide respiration. The catalytic processes of cobamide in dehalogenation processes are also discussed. Additionally, we examine OHRB, which cannot synthesize cobamide and must obtain it from the environment through a salvage pathway; the co-culture with cobamide producer is more beneficial and possible. This review aims to help readers better understand the importance and function of cobamides in reductive dehalogenation. The presented information can aid in the development of bioremediation strategies.