Bone marrow cells contribute to seven different endothelial cell populations in the heart
Parisa Shabani, Vahagn Ohanyan, Ammar Alghadeer, Daniel Gavazzi, Feng Dong, Liya Yin, Christopher Kolz, Lindsay Shockling, Molly Enrick, Ping Zhang, Xin Shi, William Chilian
TL;DR
This study shows that bone marrow cells contribute to multiple types of heart blood vessels and help improve heart function after repeated ischemia.
Contribution
The study reveals that bone marrow-derived cells form seven distinct endothelial cell types in the heart under ischemic conditions.
Findings
Repetitive ischemia improved cardiac function and increased capillary density in rats.
Bone marrow-derived endothelial cells showed an angiogenic gene expression profile.
Single-cell RNA sequencing identified seven distinct endothelial cell subpopulations in the heart.
Abstract
Understanding the mechanisms underlying vascular regeneration in the heart is crucial for developing novel therapeutic strategies for myocardial ischemia. This study investigates the contribution of bone marrow-derived cells to endothelial cell populations in the heart, and their role in cardiac function and coronary circulation following repetitive ischemia (RI). Chimeric rats were created by transplanting BM cells from GFP female rats into irradiated male recipients. After engraftment chimeras were subjected to RI for 17 days. Vascular growth was assessed from recovery of cardiac function and increases in myocardial blood flow during LAD occlusion. After sorting GFP+ BM cells from heart and bone of Control and RI rats, single-cell RNA sequencing was implemented to determine the fate of BM cells. Our in vivo RI model demonstrated an improvement in cardiac function and myocardial blood…
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Taxonomy
TopicsEngineering and Information Technology · Environmental and Ecological Studies