# osm-5p- driven fluorophores are differentially expressed in ccpp-1Δ and nekl-4Δ mutant ciliated neurons

## TL;DR

This study shows that the expression of a key ciliary protein, OSM-5, is affected in specific mutant worms, suggesting new regulatory mechanisms.

## Contribution

The study reveals that OSM-5 expression levels may be regulated by ccpp-1 and nekl-4, introducing new regulatory pathways in ciliary biology.

## Key findings

- OSM-5-driven fluorophores show altered brightness in ccpp-1Δ and nekl-4Δ mutants.
- This suggests that ccpp-1 and nekl-4 may regulate osm-5 expression in ciliated neurons.
- The osm-5 promoter is commonly used for expressing constructs in ciliated neurons.

## Abstract

Intraflagellar transport (IFT) involves the coordinated transport of molecular motors and other proteins and is required for ciliogenesis and ciliary maintenance. The
C. elegans
IFT protein
OSM-5
/IFT88 is expressed in a majority of the ciliated neurons in the animal, and

osm-5

mutants exhibit structurally defective cilia. The

osm-5

promoter is commonly used to express genetic constructs in the ciliated neurons. In this study, we show that brightness of
osm-5p-
driven constructs is altered in mutants of the tubulin deglutamylase

ccpp-1

and the NIMA-related kinase

nekl-4

. This raises the possibility that

osm-5

expression levels may be regulated by

ccpp-1

and

nekl-4

.