# Transcriptome Analysis Reveals the Mechanism of Quinoa Polysaccharides Inhibiting 3T3-L1 Preadipocyte Proliferation

## TL;DR

This study explores how quinoa polysaccharides inhibit fat cell growth by analyzing gene expression changes in preadipocytes.

## Contribution

The study identifies specific genes and pathways affected by quinoa polysaccharides in preadipocytes, offering new insights into their anti-obesity mechanism.

## Key findings

- Quinoa polysaccharides significantly alter gene expression in 3T3-L1 preadipocytes.
- 20 key biological pathways are enriched, including NOD-like receptor and PI3K-Akt signaling.
- Genes Atp13A4 and Gbgt1 show consistent upregulation and downregulation across treatment groups.

## Abstract

Quinoa is a highly nutritious and biologically active crop. Prior studies have demonstrated that quinoa polysaccharides exhibit anti-obesity activity. This investigation confirmed that quinoa polysaccharides have the ability to inhibit the growth of 3T3-L1 preadipocytes. The objective of transcriptome research was to investigate the mechanism of quinoa water-extracted polysaccharides and quinoa alkaline-extracted polysaccharides that hinder the growth of 3T3-L1 preadipocytes. There were 2194 genes that showed differential expression between untreated cells and those treated with high concentrations of quinoa water-extracted polysaccharides (QWPHs). There were 1774 genes that showed differential expression between untreated cells and those treated with high concentrations of quinoa alkaline-extracted polysaccharides (QAPHs). Through gene ontology and KEGG pathway analysis, 20 characteristic pathways are found significantly enriched between the untreated group and the QAPH and QWPH groups. These pathways include the NOD-like receptor, Hepatitis C, and the PI3K-Akt signaling pathway. Atp13A4 and Gbgt1 have been identified as genes that are upregulated and downregulated in both the untreated group and the QWPH group, as well as in the untreated group and the QAPH group. These findings establish a theoretical foundation for exploring quinoa polysaccharides as an anti-obesity agent.