# Homing and characteristic analysis of macrophage in immune-mediated aplastic anemia model mice

## TL;DR

This study tracks macrophage movement and changes in mice with immune-mediated aplastic anemia, revealing their role in disease progression.

## Contribution

The study reveals macrophage homing patterns and immune pathways in aplastic anemia using a novel fluorescent labeling and proteomic approach.

## Key findings

- Labeled macrophages migrate to lymph nodes, expand, and then move to bone marrow and spleen in AA model mice.
- Proteomic analysis identified immune and inflammatory pathways activated by macrophages in the bone marrow microenvironment.
- Dynamic changes in macrophage populations correlate with disease progression in the AA model.

## Abstract

研究免疫介导的再生障碍性贫血（AA）模型小鼠体内巨噬细胞在不同器官的动态归巢过程、特征。通过磁珠分选出供鼠淋巴结中巨噬细胞并用PKH67荧光标记，参照AA模型制备方法造模后，分析小鼠血常规、骨髓活检及HE染色结果，验证造模效果。在造模的第4、8、12天，收集骨髓、脾脏和淋巴结单个核细胞，通过流式细胞术分析PKH67荧光标志供鼠巨噬细胞的动态变化。探究PKH67荧光标志的巨噬细胞，在AA模型小鼠发病过程中的动态变化，观测到供鼠巨噬细胞归巢到淋巴结并扩增分化，最终转运至骨髓和脾脏。通过蛋白组学质谱分析，初步揭示了巨噬细胞参与AA骨髓微环境激活后的相关免疫炎症反应通路，为病理性巨噬细胞参与AA模型小鼠的发病提供了依据。