# Cell‐free DNA analysis for recurrent respiratory papillomatosis: A case report

## TL;DR

This case report shows that cell-free DNA viral load can track the progression and remission of respiratory papillomatosis caused by HPV.

## Contribution

The study demonstrates cfDNA as a potential biomarker for monitoring HPV-related disease remission.

## Key findings

- HPV type 11 was detected in tumor tissue DNA via PCR.
- Pre-surgery cfDNA viral load decreased below detection limits post-surgery, matching clinical remission.
- cfDNA viral load aligns with clinical outcomes in respiratory papillomatosi

## Abstract

A 35‐year‐old male presented with recurrent respiratory papillomatosis. Human papillomavirus type 11 was detected from all sites of tumor tissue DNA by PCR. The pre‐surgery cell‐free DNA (cfDNA) viral load (3.33 × 103 copies/ng DNA) fell below the post‐surgical detection limits on achieving remission, suggesting cfDNA's potential as a biomarker.

Charting the evolution of the human papillomavirus (HPV) viral load in cell‐free DNA (cfDNA). While viral load was detectable preoperatively, it became undetectable postoperatively, aligning with clinical observations of achieving remission.