# Sirtuin 1, as a potential prognosis marker in clear cell renal cell carcinoma, regulates lipid metabolism and immune infiltration

## TL;DR

This study shows that high levels of Sirtuin 1 in kidney cancer are linked to worse survival, fat buildup, and immune system changes.

## Contribution

The study identifies Sirt1 as a new marker for predicting prognosis in clear cell renal cell carcinoma.

## Key findings

- High Sirt1 expression correlates with worse prognosis and increased tumor necrosis in ccRCC.
- Sirt1 is linked to lipid metabolism disruption and immune infiltration in tumor tissues.
- A nomogram was developed to predict survival based on Sirt1 expression and immune infiltration.

## Abstract

Clear cell renal cell carcinoma (ccRCC) is a malignancy with a dismal prognosis, caused by the buildup of fat and glycogen. Sirtuin 1 (Sirt1) is a deacetylase that regulates lipid metabolism. In this study, we collected tumor and paracancer tissues from 386 ccRCC patients and followed their prognosis over an extended time period. The expression of Sirt1 in these tissues was assessed using immunohistochemistry, and LinkedOmics database analysis identified differentially expressed genes associated with Sirt1. The survival curve was generated using the Kaplan–Meier method, and immune infiltration was analyzed using the Tumor Immune Estimation Resource (TIMER) web tool. Our findings revealed that Sirt1 was expressed in tumor tissues, but not in normal tissues, and its high expression was associated with a worse prognosis. Furthermore, we observed a positive correlation between high Sirt1 expression and perirenal fat (PF) invasion and necrosis, leading to poorer survival outcomes. We established a nomogram to predict prognosis, and a correlation was observed with immune infiltration. In conclusion, our results suggest that high Sirt1 expression is associated with lipid metabolism disorder and immune infiltration, ultimately contributing to a dismal prognosis in ccRCC.