# Determining amino acid requirements in humans

## TL;DR

This paper reviews recent studies on amino acid requirements in children, pregnant women, and the elderly to improve dietary guidelines.

## Contribution

The paper presents new amino acid requirement data for vulnerable populations using the IAAO method.

## Key findings

- Amino acid requirements were evaluated in school-age children using the IAAO technique.
- Pregnant females' amino acid needs were assessed to inform dietary recommendations.
- Elderly amino acid requirements were studied to support evidence-based guidelines.

## Abstract

Amino acids form the building blocks of body protein. Dietary protein sources provide the amino acids needed, but protein sources vary widely in amio acid composition. To ensure humans can meet body demands for amino acids, amino acid intake recommendations are provided by the Dietary Reference Intakes (DRI) and by Food and Agriculture Organization/World Health Organization/United Nations University (FAO/WHO/UNU). Current amino acid intake recommendations, however, are based on data collected predominantly from young adult males. The development of the minimally invasive indicator amino acid oxidation (IAAO) method has permitted the evaluation of amino acid requirements in various vulnerable populations. The purpose of this review is to discuss recent amino acid requirement studies in school-age children, pregnant females and the elderly determined using the IAAO technique. These requirements will help to inform evidence-based recommendations that will help to guide dietary guidelines.