# Deposition of Human-Serum-Albumin-Functionalized Spheroidal Particles on Abiotic Surfaces: Reference Kinetic Results for Bioparticles

## TL;DR

This study examines how human serum albumin coats polymer particles and how these particles deposit on different surfaces, providing insights into bioparticle behavior.

## Contribution

The paper provides reference kinetic results for bioparticle deposition using HSA-functionalized particles and QCM.

## Key findings

- HSA corona formation on polymer particles was monitored using LDV and dynamic light scattering.
- Deposition kinetics on mica, silica, and gold sensors were analyzed under various conditions.
- The random sequential adsorption model was used to interpret results for virus and bacteria deposition.

## Abstract

Human serum albumin (HSA) corona formation on polymer microparticles of a spheroidal shape was studied using dynamic light scattering and Laser Doppler Velocimetry (LDV). Physicochemical characteristics of the albumin comprising the zeta potential and the isoelectric point were determined as a function of pH for various ionic strengths. Analogous characteristics of the polymer particles were analyzed. The adsorption of albumin on the particles was in situ monitored by LDV. The stability of the HSA-functionalized particle suspensions under various pHs and their electrokinetic properties were also determined. The deposition kinetics of the particles on mica, silica and gold sensors were investigated by optical microscopy, AFM and quartz microbalance (QCM) under diffusion and flow conditions. The obtained results were interpreted in terms of the random sequential adsorption model that allowed to estimate the range of applicability of QCM for determining the deposition kinetics of viruses and bacteria at abiotic surfaces.