# Preliminary Bioactivity Assessment of Myrothecium Species (Stachybotryaceae) Crude Extracts against Aedes aegypti (Diptera: Culicidae): A First Approach from This Phytopathogenic Fungi

## TL;DR

This study explores how extracts from three Myrothecium fungi species affect Aedes aegypti mosquito larvae, showing potential as natural insecticides.

## Contribution

It is the first study to assess the bioactivity of Myrothecium species against Aedes aegypti larvae.

## Key findings

- Ethanolic extracts of Myrothecium species showed 100% biological activity against mosquito larvae at 600 ppm after 72 hours.
- Toxic effects were observed at 800 ppm for M. dimerum and M. nivale after 48 hours, and for M. roridum after 72 hours.
- Phytochemical screening identified alkaloids, flavonoids, terpenoids, and tannins as potential bioactive compounds.

## Abstract

Mosquitoes, as insect vectors, play a crucial role in transmitting viruses and parasites, leading to millions of human deaths in tropical and subtropical regions worldwide. This study aimed to evaluate the effects of ethanolic extracts of three species within the genus Myrothecium (M. roridum, M. dimerum, and M. nivale) on Aedes aegypti mosquito larvae to assess the inhibitory effect on growth and development, as well as to determine mortality. We quantify the average lethal concentrations and provide a qualitative characterization of the chemical groups responsible for their potential. Phytochemical screening revealed the presence of alkaloids, flavonoids, and terpenoids in the ethanolic extracts of the three fungal species. Tannins were found only in the extracts of M. dimerum and M. roridum. We observed a clear dependence of the effects of the crude extracts on mosquito larvae on the concentrations used and the duration of exposure. The toxic effect was observed after 48 h at a concentration of 800 ppm for both M. dimerum and M. nivale, while M. roridum showed effectiveness after 72 h. All three species within the genus Myrothecium exhibited 100% biological activity after 72 h of exposure at 600 ppm. At lower concentrations, there was moderate growth and development inhibitory activity in the insect life cycle. The study highlights the effectiveness of crude Myrothecium extracts in combating mosquito larvae, with effects becoming apparent between 48 and 72 h of exposure. This initial approach underscores the potential of the fungus’s secondary metabolites for further in-depth analysis of their individual effects or synergies between them.