# Advances in Chromatographic Analysis of Phenolic Phytochemicals in Foods: Bridging Gaps and Exploring New Horizons

## TL;DR

This review explores recent advances in chromatographic methods for analyzing phenolic phytochemicals in foods, emphasizing improved precision and sustainability.

## Contribution

The paper provides a comprehensive overview of both conventional and advanced chromatographic techniques, highlighting innovations and standardization needs.

## Key findings

- Advanced chromatographic techniques like Hydrophilic Interaction Liquid Chromatography and Nano-LC offer improved detection capabilities.
- Standardized procedures according to FDA and ISO guidelines are crucial for reliable results.
- Sustainable practices are being developed to reduce the environmental impact of chromatographic methods.

## Abstract

Chromatographic analysis of phenolic phytochemicals in foods has significantly advanced over the past decade (2014–2024), meeting increasing demands for precision and efficiency. This review covers both conventional and advanced chromatographic techniques used for detecting phenolic phytochemicals in foods. Conventional methods like High-Performance Liquid Chromatography, Ultra High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Gas Chromatography are discussed, along with their benefits and limitations. Advanced techniques, including Hydrophilic Interaction Liquid Chromatography, Nano-LC, Multidimensional Liquid Chromatography, and Capillary Electrophoresis, are highlighted for their innovations and improved capabilities. The review addresses challenges in current chromatographic methods, emphasizing the need for standardized and validated procedures according to the Food and Drug Administration, European Cooperation for Accreditation of Laboratories, and The International Organization for Standardization guidelines to ensure reliable and reproducible results. It also considers novel strategies for reducing the environmental impact of chromatographic methods, advocating for sustainable practices in analytical chemistry.