# Telescoping a Prenyltransferase and a Diterpene Synthase to Transform Unnatural FPP Derivatives to Diterpenoids

## TL;DR

Scientists used two enzymes to create new diterpenoids from modified FPP, resulting in unique chemical structures not seen before.

## Contribution

The study reports the first observation of a 1,12-terpene cyclization and the creation of new diterpenoid structures.

## Key findings

- Four new diterpenoid products were synthesized using a prenyltransferase and a diterpene synthase.
- Three new cyclododecane cores and a macrocyclic ether were produced from non-natural FPP derivatives.
- A 1,12-terpene cyclization was observed for the first time through a shift in the double bond position.

## Abstract

New diterpenoids are accessible from non-natural FPP
derivatives
as substrates for an enzymatic elongation cyclization cascade using
the geranylgeranyl pyrophosphate synthase (GGPPS) from Streptomyces
cyaneofuscatus and the spata-13,17-diene synthase (SpS) from Streptomyces xinghaiensis. This approach led to four new
biotransformation products including three new cyclododecane cores
and a macrocyclic ether. For the first time, a 1,12-terpene cyclization
was observed when shifting the central olefinic double bond toward
the geminial methyl groups creating a nonconjugated 1,4-diene.