# Construction and Identification of Eukaryotic Expression Vector pEGFP-N1-MIC-1 for Mouse MIC-1 Gene and Its Effect on Gastric Cancer Cells

## TL;DR

This study constructs a gene expression vector for the mouse MIC-1 gene and shows it increases cancer cell migration and colony formation.

## Contribution

The novel contribution is the construction and validation of a new eukaryotic expression vector for mouse MIC-1 and its functional impact on gastric cancer cells.

## Key findings

- The pEGFP-N1-MIC-1 vector was successfully constructed and confirmed by enzyme digestion and DNA sequencing.
- Transfection with the vector increased MIC-1 protein expression and enhanced MFC cell migration and colony formation.
- The findings suggest MIC-1 may play a role in promoting gastric cancer cell behavior.

## Abstract

This study aimed to construct an eukaryotic expression vector, pEGFP-N1-MIC-1, for overexpressing the mouse macrophage inhibitory cytokine-1 (MIC-1) gene. Additionally, we transfected the MFC cell line to observe the upregulation of MIC-1 gene expression and assess its impact on macrophage phenotype conversion. Enzyme digestion and DNA sequencing confirmed the successful construction of the pEGFP-N1-MIC-1 vector. The transfected MFC cells exhibited a significant increase in MIC-1 protein expression levels. Furthermore, transfection with pEGFP-N1-MIC-1 increased the migration and colony formation capabilities of MFC cells. These results may contribute to future research and the development of therapeutic interventions targeting MIC-1 in macrophages, particularly in the context of gastric cancer.