# Pengzhenrongella frigida sp. nov., isolated from a glacier

## TL;DR

A new species of glacier-isolated bacterium, Pengzhenrongella frigida, is described based on genetic and biochemical analysis.

## Contribution

A novel species of Pengzhenrongella is proposed based on polyphasic characterization of a glacier-isolated strain.

## Key findings

- Strain HLT2-17T has 16S rRNA gene sequence similarities of 98.3% and 98.2% with P. phosphoraccumulans and P. sicca.
- Average nucleotide identity and DNA-DNA hybridization values confirm it is a distinct species.
- Phylogenomic analysis and fatty acid profile support its classification as Pengzhenrongella frigida sp. nov.

## Abstract

A Gram-stain-positive, rod-shaped bacterium, designated as HLT2-17T, was isolated from soil sample taken from the Hailuogou glacier in Sichuan province, PR China. Strain HLT2-17T was capable of growing at 4–25°C and in NaCl concentrations ranging from 0 to 2% (w/v). The highest level of 16S rRNA gene sequence similarity was observed with Pengzhenrongella phosphoraccumulans M0-14T (98.3 %) and Pengzhenrongella sicca LRZ-2T (98.2 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain HLT2-17T and its closest relatives, P. phosphoraccumulans M0-14T and P. sicca LRZ-2T, were 80.0–84.0 % and 23.3–27.7 %, respectively. Phylogenomic analysis indicated that strain HLT2-17T clustered together with strains P. phosphoraccumulans M0-14T and P. sicca LRZ-2T. Strain HLT2-17T contained C16 : 0 and anteiso-C15 : 0 as the major fatty acids, and MK-9(H4) as the menaquinone. Therefore, based on a polyphasic approach, we propose that strain HLT2-17T (=CGMCC 1.11116T= NBRC 110443T) represents a novel species of the genus Pengzhenrongella and suggest the name Pengzhenrongella frigida sp. nov.