TMT-based proteomics analysis of the blood enriching mechanism of the total Tannins of Gei Herba in mice

TL;DR

This study shows that tannins from Lanbuzheng help treat anemia in mice by boosting blood cell counts and activating specific biological pathways.

Contribution

The study reveals the blood-enriching mechanism of Lanbuzheng tannins through proteomics and signaling pathway analysis.

Findings

LBZT increased red blood cells, platelets, and white blood cells in anemic mice.

377 differentially expressed proteins were identified, with key pathways related to blood formation activated.

Therapeutic effects are linked to the complement and coagulation cascade signaling pathways.

Abstract

Lanbuzheng (LBZ) is the traditional seedling medicine in Guizhou, which has the effect of tonifying blood. It has been found that the main active ingredient is tannin, however, the blood-replenishing effect of tannin and its mechanism are still unclear.

The study was to explore the mechanisms underlying the therapeutic effects of the total Tannins of Lanbuzheng (LBZT) against anemia in mice. Anemia mice was induced by cyclophosphamide, the effect of LBZT against anemia was determined by analyzing peripheral blood and evaluating organs indexes. Tandem mass tag (TMT)-based quantitative proteomics technology coupled with bioinformatics analysis was then used to identify differentially expressed proteins (DEPs) in spleen. Compared to the model, number of RBCs, PLTs and WBCs, HCT ratio and HGB content were increased, the indexes of thymus, spleen and liver were also increased, after LBZT intervention. A total of 377 DEPs were identified in LBZT group, of which 206 DEPs were significantly up-regulated and 171 DEPs were significantly down-regulated. Bioinformatics analysis showed that hematopoietic function has been restored by activating the complement and coagulation cascade signaling pathways. Results suggest that LBZT exerts it therapeutic effects against anemia by regulating complement and coagulation cascade signaling pathways and provides scientific basis for further mechanistic studies for LBZT.

•LBZT effectively improved anemia in cyclophosphamide (CYC)-induced mice.•Mechanisms of protection involve upregulating of peripheral blood and organs indexes.•Activation of complement and coagulation cascade signaling pathways underlies the protection afforded by LBZT.

LBZT effectively improved anemia in cyclophosphamide (CYC)-induced mice.

Mechanisms of protection involve upregulating of peripheral blood and organs indexes.

Activation of complement and coagulation cascade signaling pathways underlies the protection afforded by LBZT.

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