# A Chromosome-Level Genome Assembly and Annotation for the Oecanthus rufescens (Orthoptera: Oecanthidae)

## TL;DR

This paper presents a high-quality chromosome-level genome assembly and annotation for the Rufous cricket, Oecanthus rufescens.

## Contribution

The study provides a detailed genome assembly and annotation for Oecanthus rufescens, including chromosome-level structure and gene identification.

## Key findings

- The genome size is 877.9 Mb with ten pseudo-chromosomes and high continuity (N50 of 157,110,771 bp).
- Genome annotation identified 15,031 protein-coding genes and over 310 Mb of repetitive sequences.
- BUSCO analysis showed high genome integrity with 96.8% single-copy completeness.

## Abstract

Oecanthus is a genus of cricket known for its distinctive chirping and distributed across major zoogeographical regions worldwide. This study focuses on Oecanthus rufescens, and conducts a comprehensive examination of its genome through genome sequencing technologies and bioinformatic analysis. A high-quality chromosome-level genome of O. rufescens was successfully obtained, revealing significant features of its genome structure. The genome size is 877.9 Mb, comprising ten pseudo-chromosomes and 70 other sequences, with a GC content of 41.38% and an N50 value of 157,110,771 bp, indicating a high level of continuity. BUSCO assessment results demonstrate that the genome's integrity and quality are high (of which 96.8% are single-copy and 1.6% are duplicated). Comprehensive genome annotation was also performed, identifying approximately 310 Mb of repetitive sequences, accounting for 35.3% of the total genome sequence, and discovering 15,481 tRNA genes, 4,082 rRNA genes, and 1,212 other noncoding genes. Furthermore, 15,031 protein-coding genes were identified, with BUSCO assessment results showing that 98.4% (of which 96.3% are single-copy and 1.6% are duplicated) of the genes were annotated.