Dataset on sperm quality parameters and NMR-detected changes in metabolic profile of fresh and frozen turkey spermatozoa related to two different reproductive period ages

TL;DR

This study provides data on how turkey sperm quality and metabolism change with age and freezing, offering insights for improving sperm preservation techniques.

Contribution

The dataset reveals age-related metabolic changes in cryopreserved turkey sperm, adding new insights for improving freezing protocols.

Findings

Cryopreservation significantly affected sperm quality parameters at both 44 and 56 weeks of age.

Frozen semen at 56 weeks showed more metabolic changes in water and lipid-soluble metabolites than at 44 weeks.

The dataset could help develop better freezing strategies for older turkey sperm.

Abstract

Significant changes in the quality and metabolic profile of fresh turkey sperm as a result of both cryopreservation and reproductive age have already been individually confirmed in our previous studies. This new dataset adds a relevant piece to the tangled puzzle of changes in metabolite levels affecting cryopreserved turkey sperm quality, taking into consideration two different reproductive period ages. Fresh semen samples were collected at 44 and 56 weeks of age and exposed to the cryopreservation process. All fresh and frozen-thawed samples were subjected to analysis of mobility, viability and osmotic tolerance as parameters for evaluating the sperm quality, while NMR spectroscopy was used to assess the quantitative changes in water and lipid-soluble metabolites. Our results showed that the cryopreservation process significantly affected all of the measured qualitative parameters both at 44 and 56 weeks. Concerning the metabolic profile, a greater number of quantitative changes for both water and lipid-soluble components were found in frozen semen at 56 weeks than at 44 weeks of age. These data could contribute to identifying new strategies aimed at improving freezing procedures even as reproductive age increases.

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