# Characterization of temperature-sensitive alleles of Schizosaccharomyces pombe septation initiation network components

## TL;DR

This paper characterizes temperature-sensitive mutations in key components of a cell division network in a type of yeast.

## Contribution

The study identifies and characterizes new temperature-sensitive alleles in four SIN components of Schizosaccharomyces pombe.

## Key findings

- Temperature-sensitive mutations in cdc14, cdc16, sid1, and sid2 were characterized.
- The mutations affect SIN signaling and growth at different temperatures.
- These new mutants provide tools for studying cell division mechanisms.

## Abstract

The
Schizosaccharomyces pombe
septation initiation network (SIN) promotes cytokinesis and septation. Comprised of a protein kinase cascade triggered by activation of a small GTPase and inhibited by a two-component GAP that localize to the spindle pole bodies in a cell cycle specific manner. Here, we characterized temperature-sensitive mutants isolated in the 1990s in four SIN components. We determined the mutations within each

cdc14

,

cdc16

,

sid1
,

and

sid2

mutant allele and analyzed their growth at different temperatures compared with known mutant alleles. The new mutants described here expand the toolkit for studying SIN signaling.