Comparative genomics of Tn6411 transposons carrying the blaIMP-1 gene in Pseudomonas aeruginosa

TL;DR

This study analyzes two carbapenem-resistant Pseudomonas aeruginosa strains and explores the Tn6411 transposon carrying the blaIMP-1 gene.

Contribution

The study identifies Tn6411 transposon carrying blaIMP-1 in Pseudomonas aeruginosa and highlights its potential transferability.

Findings

The Pseudomonas aeruginosa strains belong to sequence type ST865 and serotype O6.

Tn6411 transposon carrying blaIMP-1 was identified in strain 18083286.

The transposon was found in other P. aeruginosa chromosomes and Acinetobacter plasmids in GenBank.

Abstract

We aimed to determine the molecular characteristics of carbapenem-resistant Pseudomonas aeruginosa strains 18081308 and 18083286, which were isolated from the urine and the sputum of two Chinese patients, respectively. Additionally, we conducted a comparative analysis between Tn6411 carrying blaIMP-1 in strain 18083286 and transposons from the same family available in GenBank. Bacterial genome sequencing was carried out on strains 18081308 and 18083286 to obtain their whole genome sequence. Average nucleotide identity (ANI) was used for their precise species identification. Serotyping and multilocus sequence typing were performed. Furthermore, the acquired drug resistance genes of these strains were identified. The carbapenem-resistant P. aeruginosa strains isolated in the present study were of sequence type ST865 and serotype O6. They all carried the same resistance genes (aacC2, tmrB, and blaIMP-1). Tn6411, a Tn7-like transposon carrying blaIMP-1, was found in strain 18083286 by single molecule real time (SMRT) sequencing. We also identified the presence of this transposon sequence in other chromosomes of P. aeruginosa and plasmids carried by Acinetobacter spp. in GenBank, indicating the necessity for heightening attention to the potential transferability of this transposon.

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