# Evaluation of the Genotoxic Effects of Grape Seed Extract and Marine Collagen Peptide on the Fibroblast Cell Line: An In Vitro Study

## TL;DR

This study tested grape seed extract and marine collagen peptides on dental cells and found both to be safe with minimal DNA damage.

## Contribution

The study provides new in vitro evidence on the biocompatibility of grape seed extract and marine collagen peptides with dental pulp cells.

## Key findings

- GSE showed minimal cell death compared to marine collagen peptides.
- Viable cell counts increased over three days in all groups.
- Control group had the highest viable cell count, with significant differences among groups.

## Abstract

Introduction

Collagen plays a vital role in maintaining the structural integrity of dentin, and its modification with bioactive compounds can enhance its mechanical properties and bonding capabilities.

Aim

This study aimed to evaluate the genotoxic effects of grape seed extract (GSE) and marine collagen peptide (MCP) on dental pulp-derived primary cells.

Methodology

Human dental pulp stem cells were isolated, cultivated, and then treated with GSE and marine collagen peptides. DNA fragmentation was assessed using DAPI (4′,6-diamidino-2-phenylindole) staining. Statistical analysis was performed using SPSS version 20 (IBM Corp., Armonk, NY, USA).

Results

The results showed that GSE exhibited a minimum level of cell death compared to marine collagen peptides. The viable cell count increased steadily over three days in all groups, with the control group showing the highest number of viable cells. The differences in viable cell count among the groups were statistically significant.

Conclusion

This study suggests that GSE and marine collagen peptides are highly biocompatible with dental pulp cells and could be considered for further clinical studies.