# Expression analysis and biological regulation of silencing regulatory protein 6 (SIRT6) in cutaneous squamous cell carcinoma

## TL;DR

This study explores how SIRT6 protein affects the progression of skin cancer and finds that reducing SIRT6 can slow cancer growth and spread.

## Contribution

The study reveals SIRT6's role in CSCC progression and its potential as a therapeutic target.

## Key findings

- High SIRT6 expression correlates with cancer location and staging in CSCC patients.
- SIRT6 silencing reduces CSCC cell proliferation, migration, and invasion while promoting apoptosis.
- SIRT6 knockdown blocks CSCC cells in the G1 phase of the cell cycle.

## Abstract

Cutaneous squamous cell carcinoma (CSCC) is one of the most common types of skin cancer worldwide. Therefore, the identification of biomarkers associated with CSCC progression could aid in the early detection of high-risk squamous cell carcinoma and the development of novel therapeutic strategies.

This study aimed to investigate the expression patterns of silent mating type Information Regulation 2 homolog 6 (SIRT6) in CSCC and its clinical significance.

The protein expression level of SIRT6 in tissues was detected by immunohistochemistry, and the correlation between SIRT6 expression and clinicopathological parameters in CSCC patients was analyzed. The relative expression of SIRT6 in CSCC cell lineage and tissue specimens was determined by western blotting and PCR. The effect of SIRT6 silencing on cell proliferation was evaluated using cell counting kit 8. Wound healing, transwell method, and flow cytometry were used to investigate the migration, invasion, and cell cycle distribution/apoptosis of CSCC cells after SIRT6 silencing, respectively. Western blot was used to detect the expression of EMT (Epithelial-Mesenchymal Transition), cycle, apoptosis, and other related proteins.

The high expression of SIRT6 was correlated with the location of cancer tissue and Broder staging in CSCC patients. Knockdown of SIRT6 inhibited the proliferation, migration, invasion and EMT of CSCC cells, and promoted their apoptosis, with cells blocked in G1 phase.

No animal experiments were conducted to further verify the results.

Decreased expression of SIRT6 can inhibit the occurrence and development of CSCC.