Diagnostic Usefulness of Liquid Culture Medium for Mycobacterium avium-intracellulare Complex Lung Disease: A Single-Centre, Retrospective Study
Hiroshi Kobe, Akihiro Ito, Yosuke Nakanishi, Yui Miyazaki, Hiroshi Takahashi, Yushi Toyota, Akihiko Amano, Kyoko Matsui, Tadashi Ishida
TL;DR
This study shows that using liquid culture improves the early diagnosis of Mycobacterium avium-intracellulare complex lung disease compared to traditional solid culture methods.
Contribution
The study demonstrates the diagnostic superiority of liquid culture over Ogawa culture for MAC-LD in a large retrospective cohort.
Findings
Liquid culture had a 98.3% positivity rate compared to 44.9% for Ogawa culture.
75.5% of patients would have experienced delayed diagnosis without liquid culture.
25.3% of smear-positive specimens were positive only on liquid culture.
Abstract
Background The diagnosis of Mycobacterium avium-intracellulare complex lung disease (MAC-LD) requires two or more positive sputum cultures. Few reports have examined the usefulness of adding liquid culture to conventional solid culture for diagnosing MAC-LD. Methods A retrospective, cohort study of patients examined at Kurashiki Central Hospital in Japan with a confirmed diagnosis of MAC-LD between January 1, 2002, and June 20, 2021, was conducted. The primary endpoint was the culture positivity rate, which was compared between the liquid and Ogawa culture media in patients who underwent sputum culture using both methods. Secondary endpoints were the culture positivity rate in smear-positive specimens and the positivity rate by radiological type. Results The study, which involved 351 patients and 702 specimens, showed a higher positivity rate for liquid culture (n=690, 98.3%) than…
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Taxonomy
TopicsMycobacterium research and diagnosis · Infectious Diseases and Mycology · Tuberculosis Research and Epidemiology