# Characterization of temperature-sensitive Schizosaccharomyces pombe mutants in the septation initiation network Spg1 GTPase

## TL;DR

This study examines temperature-sensitive mutations in the Spg1 GTPase of fission yeast, which is crucial for cell division.

## Contribution

The paper provides a detailed characterization of temperature-sensitive spg1 alleles and their interactions with other SIN alleles.

## Key findings

- Temperature-sensitive spg1 alleles were identified and their mutations characterized.
- Growth defects of these alleles were assessed under different conditions.
- Interactions between the spg1 alleles and other SIN alleles were evaluated.

## Abstract

The
Schizosaccharomyces pombe
GTPase,
Spg1
, activates the septation initiation network (SIN) protein kinase cascade to trigger septation. In the absence of functional
Spg1
, cells fail cytokinesis and become multinucleate. In this study, we characterize a set of temperature-sensitive

spg1

alleles isolated in the 1990s. We identify the mutations within each new and previously characterized allele, characterize the extent of relative growth defects, and assess their interaction with other SIN alleles.