# Identification of the New GmJAG1 Transcription Factor Binding Motifs Using DAP-Seq

## TL;DR

This study identifies new DNA binding motifs for the GmJAG1 transcription factor in soybean, which helps regulate leaf shape, seed number, and flower size.

## Contribution

The study discovers and experimentally validates two novel GmJAG1 binding motifs using DAP-seq and EMSA.

## Key findings

- Two new GmJAG1 binding motifs were identified using DAP-seq.
- EMSA experiments confirmed GmJAG1's binding to these motifs.
- Potential regulatory network mediated by GmJAG1 was constructed.

## Abstract

Interaction between transcription factors (TFs) and motifs is essential for gene regulation and the subsequent phenotype formation. Soybean (Glycine max) JAGGEED 1 (GmJAG1) is a key TF that controls leaf shape, seed number and flower size. To understand the GmJAG1 binding motifs, in this study, we performed the GmJAG1 DNA affinity purification sequencing (DAP-seq) experiment, which is a powerful tool for the de novo motif prediction method. Two new significant GmJAG1 binding motifs were predicted and the EMSA experiments further verified the ability of GmJAG1 bound to these motifs. The potential binding sites in the downstream gene promoter were identified through motif scanning and a potential regulatory network mediated by GmJAG1 was constructed. These results served as important genomic resources for further understanding the regulatory mechanism of GmJAG1.