# Hydrogen-Rich Alkaline Water Supplementation Restores a Healthy State and Redox Balance in H2O2-Treated Mice

## TL;DR

Hydrogen-rich alkaline water helps reduce oxidative stress and DNA damage in mice exposed to hydrogen peroxide.

## Contribution

This study demonstrates the preventive and curative effects of hydrogen-rich alkaline water on systemic redox imbalance.

## Key findings

- Hydrogen-rich alkaline water reduced plasma ROS levels and increased glutathione.
- It decreased DNA damage markers like 8-hydroxy-2′-deoxyguanosine in the blood.
- Spleen and bone marrow cells showed reduced ROS and improved mitochondrial function.

## Abstract

Water is a major requirement for our bodies, and alkaline water has induced an antioxidant response in a model of natural aging. A series of recent reports have shown that aging is related to reduced water intake. Hydrogen-rich water has been suggested to exert a general antioxidant effect in relation to both improving lifestyle and preventing a series of diseases. Here, we wanted to investigate the effect of the daily intake of hydrogen-rich alkaline water (HAW) in counteracting the redox imbalance induced in a model of H2O2-treated mice. Mice were treated with H2O2 for two weeks and either left untreated or supplied with HAW. The results show that HAW induced a reduction in the ROS plasmatic levels that was consistent with the increase in the circulating glutathione. At the same time, the reduction in plasmatic 8-hydroxy-2′-deoxyguanosine was associated with reduced DNA damage in the whole body. Further analysis of the spleen and bone marrow cells showed a reduced ROS content consistent with a significantly reduced mitochondrial membrane potential and superoxide accumulation and an increase in spontaneous proliferation. This study provides evidence for a clear preventive and curative effect of HAW in a condition of systemic toxic condition and redox imbalance.