Whole Genome Analysis and Assessment of the Metabolic Potential of Streptomyces carpaticus SCPM-O-B-9993, a Promising Phytostimulant and Antiviral Agent

TL;DR

This paper analyzes the genome of Streptomyces carpaticus SCPM-O-B-9993, a bacteria with potential to produce antiviral and plant-stimulating compounds.

Contribution

The study reveals the genome structure and metabolic potential of a novel Streptomyces strain with antiviral and phytostimulatory properties.

Findings

The genome of Streptomyces carpaticus SCPM-O-B-9993 contains biosynthetic gene clusters for antimicrobial secondary metabolites.

The strain exhibits strong phytostimulatory and antiviral effects against cucumber mosaic virus in tomatoes.

Optimal cultivation occurs at 72 hours, transitioning from exponential to stationary growth phase.

Abstract

Natural microorganism-derived products are an essential source of valuable pharmaceuticals and agrichemicals. Streptomyces spp. are the most environmentally abundant bacteria that are capable of producing various valuable natural products exhibiting significant biological activity to be used in such industries as medicine, environmental science, food industry, and agriculture. However, many natural products among Streptomyces bacteria remain unstudied. Because of the rapid increase in antimicrobial and pesticide resistance, it is currently extremely topical to develop novel antibiotics, antiviral agents, agrichemicals, and other substances. The modern methods of molecular biology allow one to detect antimicrobial resistance gene clusters in the bacterial cell. Our study detected antibiotics and terpenes in cells of investigated bacteria; these compounds are of significant interest for the plant-growing sector.

This work aimed to study the genome organization and the metabolic potential of Streptomyces carpaticus strain SCPM-O-B-9993, a promising plant-protecting and plant-stimulating strain isolated from brown semi-desert soils with very high salinity. The strain genome contains a linear chromosome 5,968,715 bp long and has no plasmids. The genome contains 5331 coding sequences among which 2139 (40.1%) are functionally annotated. Biosynthetic gene clusters (BGCs) of secondary metabolites exhibiting antimicrobial properties (ohmyungsamycin, pellasoren, naringenin, and ansamycin) were identified in the genome. The most efficient period of SCPM-O-B-9993 strain cultivation was 72 h: during this period, the culture went from the exponential to the stationary growth phase as well as exhibited excellent phytostimulatory properties and antiviral activity against the cucumber mosaic virus in tomatoes under laboratory conditions. The Streptomyces carpaticus SCPM-OB-9993 strain is a biotechnologically promising producer of secondary metabolites exhibiting antiviral and phytostimulatory properties.

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