# Transcriptional activation of candidate paused genes after partial hepatectomy

**Authors:** Franck Mayeux, Nicolas Buisine, Olivier Fayol, Patricia Uguen

PMC · DOI: 10.17912/micropub.biology.001160 · microPublication Biology · 2024-06-10

## TL;DR

This study shows that certain genes become rapidly active after liver injury, due to a release of paused RNA polymerase.

## Contribution

The paper identifies 23 candidate paused genes that are rapidly activated after partial hepatectomy.

## Key findings

- 23 genes with paused RNA polymerase were identified as candidates for rapid activation after liver injury.
- RT-qPCR confirmed quick induction of Jun, Fos, and Btg2 after partial hepatectomy.
- Hyperphosphorylation of RNA Pol II CTD Ser2 indicates release from transcriptional pause.

## Abstract

Partial hepatectomy is a model of acute liver injury that is known to induce a strong reprogrammation of gene expression. Transcriptional induction of Immediate Early Genes is extremely fast and this would be due to the release of RNA Polymerase II poised for elongation at ‘paused’ genes. Using bioinformatic analysis, we identified 23 genes sharing features of paused genes before hepatectomy, and with predicted quick and strong expression induction after. This transcriptional dynamic, confirmed by RT-qPCR for
Jun
,
Fos
,
Btg2,
is very precocious. RNA Pol II CTD Ser2 hyperphosphorylation indicates a switch to productive elongation and release from transcriptional pause.

## Linked entities

- **Genes:** JUN (Jun proto-oncogene, AP-1 transcription factor subunit) [NCBI Gene 3725], FOS (Fos proto-oncogene, AP-1 transcription factor subunit) [NCBI Gene 2353], BTG2 (BTG anti-proliferation factor 2) [NCBI Gene 7832]
- **Proteins:** RNA polymerase II (DNA-directed RNA polymerase II subunit RPB7), Polr2A (RNA polymerase II subunit A)

## Full-text entities

- **Genes:** JUN (Jun proto-oncogene, AP-1 transcription factor subunit) [NCBI Gene 3725] {aka AP-1, AP1, c-Jun, cJUN, p39}, BTG2 (BTG anti-proliferation factor 2) [NCBI Gene 7832] {aka APRO1, PC3, TIS21}, FOS (Fos proto-oncogene, AP-1 transcription factor subunit) [NCBI Gene 2353] {aka AP-1, C-FOS, p55}
- **Diseases:** acute liver injury (MESH:D017114)

## Full text

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## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC11196992/full.md

## References

19 references — full list in the complete paper: https://tomesphere.com/paper/PMC11196992/full.md

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Source: https://tomesphere.com/paper/PMC11196992