# Study on the material basis and immunological enhancement activity of dangdi oral liquid

## TL;DR

This study identifies compounds in Dangdi oral liquid and shows it boosts immune function in mice.

## Contribution

The study identifies 51 compounds in Dangdi oral liquid and demonstrates its immune-enhancing effects in mice.

## Key findings

- DDO significantly promotes lymphocyte proliferation and macrophage phagocytic ability.
- The medium dose of DDO showed the best immune-enhancing efficacy (P＜0.05).
- DDO increases hemolytic plaque number, sIgA content, and immune organ index.

## Abstract

Studies have shown that a lot of traditional Chinese medicines could improve the immunity of the body. Dangdi oral liquid (DDO) was mainly composed of Angelica sinensis (Oliv.) Diels (Danggui), Rehmannia glutinosa Libosch. (Dihuang), Achyranthes bidentata Bl. (Niuxi), Glycyrrhiza uralensis Fisch. (Gancao). In this study, the rapid ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) method was used to identify the potentially effective compounds of DDO. Then the immune activity of DDO was measured by lymphocyte proliferation, macrophage phagocytic function, NK cell activity, delayed type hypersensitivity reaction, hemolytic plaque number, sIgA content and immune organ index. The results showed that a total of 51 compounds were identified. In addition, DDO could significantly promote the lymphocyte proliferation, improve macrophage phagocytic ability, NK cell activity, hemolytic plaque number, sIgA content and immune organ index compared with control group, and the medium dose possessed the best efficacy (P＜0.05). These results indicated that DDO could enhance the immunity of mice.