Draft genome sequence data on Bacillus safensis U41 isolated from soils of Santiniketan, India

TL;DR

This paper presents the draft genome sequence of Bacillus safensis U41, isolated from soil in India, highlighting its potential for producing cellulase enzymes.

Contribution

The novel contribution is the first draft genome sequence of Bacillus safensis U41 from Santiniketan soils, India.

Findings

The genome has a size of 3,732,407 bp with an average GC content of 41.43%.

Genome annotation identified 3783 coding sequences, 64 tRNA genes, and 3 rRNA genes.

Abstract

The draft genome sequence of an isolate of Bacillus safensis U41 from the soils of Santiniketan (23040′12″ N and 87039′52″ E) is reported here. Bacillus safensis is a bacterium that produces cellulases, which is essential for the breakdown of plant biomass. As such, it is a valuable source of digestive enzymes from plant biomass, especially cellulases. The genomic DNA was extracted from a single colony using a QIAgen Blood and Tissue kit (QIAgen Inc., Canada). Sequencing was performed via Illumina HiSeq X using 2 × 150 paired-end chemistry, generating 7,352,576 reads with sequence coverage of 509x. The assembly produced 20 contigs over 200 base pairs (bp) in length, with an N50 value of 901304 and an L50 of 2. The genome size was 3,732,407 bp, and the average GC content was 41.43 %. Genome annotation and gene predictions were performed using Prokka v.1.14.6, which identified 3783 coding sequences, 64 tRNA genes, and 3 rRNA genes.

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