Rapid Analysis of Seven Polyamines in Nephotettix cincticeps by Using Ultra-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry

TL;DR

This study developed a fast and accurate method to detect seven polyamines in the insect Nephotettix cincticeps using advanced mass spectrometry.

Contribution

A novel rapid UPLC-3Q-MS method for polyamine analysis in insects without derivatization.

Findings

The method detected seven polyamines in 5 minutes with high sensitivity and precision.

Limits of detection and quantitation ranged from 24–139 pg/mg and 82–464 pg/mg, respectively.

Recovery rates were between 86–115% with low RSD values for both intraday and interday tests.

Abstract

A fast, simple, and sensitive method for the simultaneous determination of seven polyamines in Nephotettix cincticeps was developed based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-3Q-MS) together with liquid phase extraction. Polyamines in insect samples were extracted with HClO4 solution and then were separated and detected by UPLC-3Q-MS, which was equipped with a hydrophilic interaction liquid chromatography column, within 5 min without any derivatization procedure. The method has been successfully used to detect 7 polyamines in healthy and difluormethylornithine-treated adults of Nephotettix cincticeps with a method limit of detection and the method limit of quantitation of 24–139 pg/mg and 82–464 pg/mg, respectively, an intraday and interday relative standard deviation (RSD, n = 5) of 1.97–6.00% and 2.08–5.92% respectively, and a recovery of 86–115%. The success of this study provided a reliable method for the rapid and high-throughput detection of polyamines in the insect sample.

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