# An oral toxicity assessment of a mosquito larvicidal transgenic algae (Chlamydomonas reinhardtii) using adult Zebrafish and its embryos

## TL;DR

This study shows that genetically modified algae designed to kill mosquito larvae do not harm zebrafish or their embryos.

## Contribution

The novelty lies in demonstrating the safety of transgenic algae for non-target aquatic species like zebrafish.

## Key findings

- No significant differences in mortality, allergenicity, or moribundity were observed in zebrafish fed with transgenic algae.
- Hematology and molecular analysis showed no physiological differences in zebrafish exposed to the algae.
- The transgenic algae (TN72.cry11Ba) had no adverse effects on zebrafish or their larvae.

## Abstract

Mosquito-borne diseases pose a global health threat, with pathogens like Malaria, Dengue fever, and others transmitted by mosquitoes. Our study focuses on evaluating the toxicity of genetically engineered mosquito larvicidal algae (Chlamydomonas reinhardtii) to non-target organisms, specifically Zebrafish. We conducted a 90-day experiment, feeding Zebrafish different combinations of larvicidal algae and commercial fish feed. Statistical analysis revealed no significant differences in mortality, allergenicity, or moribundity among groups. Hematology, molecular analysis, and necropsy showed no physiological differences. Our findings indicate that the transgenic algae (TN72.cry11Ba) had no adverse effects on adult Zebrafish or their larvae. This study confirmed the safety of algae on non-target organisms, such as zebrafish.