# Universally Quantitative Band-Selective Pure Shift NMR Spectroscopy

## TL;DR

This paper introduces a new method to improve the accuracy of NMR spectroscopy by correcting signal loss in high-resolution experiments.

## Contribution

A novel method to correct site-dependent signal loss in band-selective pure shift NMR experiments.

## Key findings

- The method allows integrals within 1% of pulse-acquire experiments for a three-component mixture.
- It works in both interferogram and semi-realtime acquisition modes.

## Abstract

NMR spectroscopy is often described as a quantitative
analytical
technique. Strictly, only the simple pulse-acquire experiment is universally
quantitative, but the poor signal resolution of the 1H
NMR pulse-acquie experiment frequently complicates quantitative analysis.
Pure shift NMR techniques provide higher resolution, by reducing signal
overlap, but they are susceptible to a variety of sources of site-dependent
signal loss. Here, we introduce a new method that corrects for signal
loss from such sources in band-selective pure shift NMR experiments,
by performing different numbers of iterations of the same pulse sequence
elements before acquisition to allow extrapolation back to the loss-free
signal. We apply this method to both interferogram and semi-realtime
acquisition modes, obtaining integrals within 1% of those acquired
from a pulse-acquire experiment for a three-component mixture.