Appendiceal neoplasms derived from appendiceal tip remnants following appendectomy: a report of two cases

TL;DR

This paper reports two rare cases of appendiceal tumors that developed from leftover parts of the appendix after incomplete appendectomy surgeries.

Contribution

The study highlights the risk of neoplasms arising from appendiceal tip remnants, not just the stump, following incomplete appendectomy.

Findings

A 71-year-old man developed a low-grade appendiceal mucinous neoplasm from an appendiceal tip remnant.

A 65-year-old man was diagnosed with mucinous adenocarcinoma from a remnant appendiceal tip after incomplete appendectomy.

Both cases emphasize the need for postoperative monitoring when appendectomy is incomplete due to severe inflammation.

Abstract

Neoplasms derived from remnant appendix are rarely described, with most cases arising from the appendiceal “stump”. Here, we present two surgical cases of appendiceal neoplasms derived from appendiceal “tip” remnants.

The first patient was a 71-year-old man who had undergone laparoscopic appendectomy for acute appendicitis 12 years prior. During appendectomy, the appendiceal root was ligated, but the appendix was not completely removed due to severe inflammation. At the most recent presentation, computed tomography (CT) was performed to examine choledocholithiasis, which incidentally revealed a cystic lesion of approximately 90 mm adjacent to the cecum. A retrospective review revealed that the cystic lesion had increased in size over time, and laparoscopic ileocecal resection was performed. Pathology revealed no continuity from the appendiceal orifice to the cyst, and a diagnosis of low-grade appendiceal mucinous neoplasm (LAMN) was made from the appendiceal tip remnant. The patient was discharged without complications. The second patient was a 65-year-old man who had undergone surgery for peritonitis due to severe appendicitis 21 years prior. During this operation, the appendix could not be clearly identified due to severe inflammation; consequently, cecal resection was performed. He was referred to our department with a chief complaint of general fatigue and loss of appetite and a cystic lesion of approximately 85 mm close to the cecum that had increased over time. CT showed irregular wall thickening, and malignancy could not be ruled out; therefore, laparoscopic ileocecal resection with D3 lymph node dissection was performed. The pathological diagnosis revealed mucinous adenocarcinoma (TXN0M0) arising from the remnant appendiceal tip. The patient is undergoing follow-up without postoperative adjuvant chemotherapy, with no evidence of pseudomyxoma peritonei or cancer recurrence for 32 months postoperatively.

If appendicitis-associated inflammation is sufficiently severe that accurate identification of the appendix is difficult, it may remain on the apical side of the appendix, even if the root of the appendix is ligated and removed. If the appendectomy is terminated incompletely, it is necessary to check for the presence of a residual appendix postoperatively and provide appropriate follow-up.

Figures

Retrospective review of the changes over time in the appendiceal tumor on CT images (Case 1). a The appendiceal tip could be identified just before appendectomy. b An appendiceal tip remnant was suspected one month after appendectomy (19 × 19 mm). c The tip remnant had shrunk six years after appendectomy (15 × 10 mm). d The tip remnant had enlarged and formed a cyst seven years after appendectomy (35 × 25 mm). e The cystic lesion had grown more 12 years after appendectomy (92 × 45 mm)Fig. 2Pathological findings of case 1. a, b Macroscopic view of the specimen. The cyst was firmly adhered to th

Changes of CT findings overtime in case 2. a, b A cystic lesion (55*22mm) adjacent to the ileocecum was identified 19 years after appendectomy. c, d The cystic lesion was enlarged (85*35mm) with irregular wall thickening (arrowheads) 21 years after appendectomy

Pathological findings in case2. a Macroscopic view of the specimen. The cancer in the cyst wall had invaded the mucosal layer of the ileum (arrow). b The cystic lesion and cecum were adhered but not continued (H.E. × 5). c Cancer invasion of the mucosal layer of the ileum (arrows) (H.E. × 10). d Intraepithelial dysplasia (arrowheads) was observed in the cystic lesion, but not in the ileal mucosa; therefore, the adenocarcinoma was considered to have originated from the cystic lesion. Cancer invasion into the stromal tissue of the cystic lesion is also observed (H.E. × 100, enlarged view of the

Full text

Background

In rare cases, the residual appendix left after appendectomy can cause inflammation or neoplastic transformations. Although residual appendicitis and tumors derived from the residual appendix are rare, they should not be completely ruled out in patients with a history of appendectomy. An inappropriate appendectomy can leave a long-segment stump, which can cause stump appendicitis [1, 2]. More rarely, there have been several reports on obstructive mucus retention in the lumen of the remnant appendix, forming appendiceal mucinous lesions [3–5]. As described in these reports, residual appendiceal tumors are most commonly derived from an inappropriately left appendiceal stump.

Inflammations or neoplastic mucinous lesions derived from appendiceal tip remnants are even rarer. It has been reported as lesions that are not continuous with the appendiceal root or cecum. And it was described that they were derived from tip remnants due to their locations or morphologies, pathological diagnoses, or initial surgical reports [6–11]. However, the rationales for the diagnoses as being derived from the tip remnants are often unclear. Moreover, there are no reports which described their changes over time since the initial appendectomy. Herein, we present two cases of appendiceal neoplasms derived from tip remnants after appendectomy, with a detailed clinical and pathological examination.

Case presentation

Case 1

A 71-year-old man was referred to our department because computed tomography (CT) performed for choledocholithiasis incidentally revealed a large cystic lesion approximately 90 mm in size adjacent to the cecum. The patient had undergone a laparoscopic appendectomy at our hospital 12 years prior. Soon after the appendectomy, the patient underwent examination for sleep apnea syndrome (SAS). Further, he had a history of total gastrectomy for early gastric cancer 6 years after the original appendectomy.

By reviewing the first surgical report, we found that the appendiceal root was ligated and detached; however, appendectomy was insufficiently performed because of severe inflammation. We retrospectively reviewed a computed tomography (CT) scan performed one month after appendectomy for the examination of SAS, and a remnant appendiceal tip was suspected. Six years after the appendectomy, a preoperative CT scan for gastric cancer showed that the remnant appendix has shrunk, and during the surgery for the gastric cancer, no specific findings were observed except for adhesions between the cecum and the abdominal wall. Subsequent postoperative follow-up CT revealed that the appendiceal remnant had gradually increased seven years after appendectomy and had transformed into a 92*45 mm cystic lesion (Fig. 1). We suspected an appendiceal mucinous lesion arising from the tip remnant and performed surgery. Laparoscopic observation revealed no perforation or mucus dissemination; however, the tumor and ileocecum were firmly adhered and difficult to detach. Therefore, a small incision was made in the umbilical region to perform ileocecal resection. The resected specimen showed strong adhesion between the cystic lesion and the cecum, but no continuity from the appendiceal orifice to the cyst. The cyst was found to contain white viscous mucus. Pathological findings showed that the cyst wall contained columnar epithelial and muscular tissues. Based on the medical history and pathological findings, we diagnosed the patient with a low-grade appendiceal mucinous neoplasm (LAMN) arising from an appendiceal tip remnant (Fig. 2). The patient had no postoperative complications and was discharged 7 days postoperatively.Fig. 1. Retrospective review of the changes over time in the appendiceal tumor on CT images (Case 1). a The appendiceal tip could be identified just before appendectomy. b An appendiceal tip remnant was suspected one month after appendectomy (19 × 19 mm). c The tip remnant had shrunk six years after appendectomy (15 × 10 mm). d The tip remnant had enlarged and formed a cyst seven years after appendectomy (35 × 25 mm). e The cystic lesion had grown more 12 years after appendectomy (92 × 45 mm)Fig. 2. Pathological findings of case 1. a, b Macroscopic view of the specimen. The cyst was firmly adhered to the cecum, and its contents comprised white viscous mucus. There was no continuity between the cystic cavity and the cecal lumen. c A muscular layer was observed in the cyst wall, and mucus was present in the cavity. Most of the epithelial tissue of the cyst had fallen off, but neoplastic changes were observed in the epithelial tissue (hematoxylin–eosin stain (H.E.) × 20). d High columnar tumor cells with spindle-shaped enlargement of the nucleus were proliferated, and low-grade appendiceal mucinous neoplasm was observed (H.E. × 400, enlarged image of yellow square in c)

Case 2

A 65-year-old man visited our hospital with the chief complaint of general fatigue and loss of appetite, and was referred to our department for a tumor located in the ileocecal region. He had a surgical history of peritonitis caused by appendicitis 21 years prior. We inquired about the surgical report of the previous appendectomy, which revealed that the surgeons could not clearly recognize the entire appendix due to severe inflammation and performed open cecum resection. A contrast-enhanced CT scan revealed a cystic lesion 55 mm in size two years prior, which had grown to 85 mm in size with irregular thickening in part of the wall (Fig. 3). Tumor markers were also elevated (carcinoembryonic antigen (CEA): 83.5ng/ml, Carbohydrate antigen 19–9:260 ng/ml), and malignancy could not be ruled out. As such, surgery was performed. Laparoscopic investigation revealed that the tumor had ruptured and disseminated mucus around the cystic lesion; therefore, the patient was diagnosed as pseudomyxoma peritonei (PMP). Part of the tumor seemed to have invaded the ileum; therefore, we performed laparoscopic ileocecal resection with D3 lymph node dissection, followed by intraperitoneal lavage with 5000 mL of massive saline solution.Fig. 3. Changes of CT findings overtime in case 2. a, b A cystic lesion (5522mm) adjacent to the ileocecum was identified 19 years after appendectomy. c, d The cystic lesion was enlarged (8535mm) with irregular wall thickening (arrowheads) 21 years after appendectomy

Examination of the resected specimen revealed that part of the tumor had invaded the ileum and extended into the mucosal layer. Immunostaining suggested the presence of desmin-positive smooth muscle and CD56- and calretinin-positive nerve cells, indicating that the cyst wall had a normal intestinal wall structure. Mucinous and well-differentiated adenocarcinomas were observed in the cyst walls (Fig. 4). No apparent cellular components were found in the mucus (acellular mucin). The cystic lesion did not continue with the stapler or the intestinal lumen; therefore, we diagnosed it as a mucinous adenocarcinoma (TXN0M0) arising from an appendiceal tip remnant. As hepatocellular carcinoma was also found postoperatively, the patient was followed-up without adjuvant chemotherapy; however, no recurrence of PMP or appendiceal cancer was observed for 32 months postoperatively. Fig. 4. Pathological findings in case2. a Macroscopic view of the specimen. The cancer in the cyst wall had invaded the mucosal layer of the ileum (arrow). b The cystic lesion and cecum were adhered but not continued (H.E. × 5). c Cancer invasion of the mucosal layer of the ileum (arrows) (H.E. × 10). d Intraepithelial dysplasia (arrowheads) was observed in the cystic lesion, but not in the ileal mucosa; therefore, the adenocarcinoma was considered to have originated from the cystic lesion. Cancer invasion into the stromal tissue of the cystic lesion is also observed (H.E. × 100, enlarged view of the yellow square in c)

Discussion

The frequency of residual appendicitis after appendectomy is reported to be 0.06%–0.25% of all appendectomies [12], and only ten or more cases of appendiceal mucinous lesions derived from the residual appendix have previously been reported [11]. Histologically, appendiceal mucinous lesions are classified as either non-neoplastic or neoplastic appendiceal mucinous lesions. Non-neoplastic appendiceal mucinous lesions include simple mucoceles or retention cysts without epithelial neoplasia. Neoplastic appendiceal mucinous lesions include serrated polyps, hyperplastic polyps, low-grade appendiceal mucinous neoplasms (LAMN), high-grade appendiceal mucinous neoplasms (HAMN), and mucinous adenocarcinomas. Although there has been some controversy regarding the classification of appendiceal mucinous neoplasms, the Peritoneal Surface Oncology Group International (PSOGI) consensus or World Health Organization (WHO) 2019 classification does not recommend the use of confusing terms such as mucinous cystadenoma or mucinous cystadenocarcinoma, as previously reported [13, 14]. Indeed, the term mucinous adenocarcinomas should only be used if accompanied by infiltrative invasion, and should be classified without histologically destructive features: low-grade epithelial dysplasia as LAMN, and high-grade epithelial dysplasia as HAMN. Appendiceal mucinous lesions have poor prognosis if they become cancerous or rupture and progress to PMP [4, 15–20]. In a prior report, Tomida et al. reviewed 10 cases of mucinous lesions derived from the residual appendix that developed 10–40 years after appendectomy [11]; however, changes in tumors over time were not described in these reports. In our first case, we observed CT images taken for other diseases. It is interesting to note that the change in the size of the remnant appendix, which shrunk after the inflammation subsided, increased over time 7 years postoperatively.

Residual appendicitis or appendiceal tumors reported in previous studies are largely derived from the appendiceal stump [11, 12, 21]; those derived from appendiceal tip remnants are very rare. To the best of our knowledge, there have been nine reported cases, including the two reported here (Table 1). The incidence of residual tip appendicitis ranged from three months to ten years after the initial surgery. With regard to tumor development from the residual appendiceal tip, none developed cancer, and the time to occurrence ranged from 9 to 24 years after the initial surgery. Regarding the reasons for determining that the appendiceal tip remnant was primary, one report described it as having a separate mesentery [6]; however, few reports have described it in detail [7–11].Table 1. Summary of patients who had inflammations or mucinous neoplasms derived from appendiceal tip remnantsAuthorYearAgeSexSymptomDiagnosisInterval time (years)Diameter (cm)OperationJohnson et al. [6]200667MaleRLQ painMucinous cystadenoma157Tumor excisionO'Leary et al. [7]201043MaleRUQ painResidual tip appendicitis102.5Tumor excisionParthsarathi et al. [8]201713MaleLower abdominal pain, vomiting, feverResidual tip appendicitis3 months4Tumor excisionBoardman et al. [9]201950MaleRLQ painResidual tip appendicitis12Tumor excisionDjelil et al. [10]202041FemaleAbdominal painMucocele, pseudomyxoma peritonei2415UnknownDjelil et al. [10]202059MaleAbdominal painPseudomyxoma peritonei9N/AUnknownTomida et al. [11]202048MaleRLQ painMucus-filled lesion2312Ileocecal resectionOur case202471MaleNoneLow-grade appendiceal mucinous neoplasm129Ileocecal resectionOur case202465MaleGeneral fatigue, loss of appetiteMucinous adenocarcinoma218.5Ileocecal resectionRLQ: right lower quadrant, RUQ: right upper quadrant, N/A: not available

There is no disagreement regarding the diagnosis of a tumor derived from the remnant appendix if it is histologically or macroscopically confirmed that the tumor develops with continuity from the cecum or root of the appendix. However, if there is no continuity, whether the tumor is derived from the remnant appendix may be questioned. If there was no continuity with the root of the appendix, three differential diagnoses can be considered. The first is when the distal side of the appendix is left and the tumor develops. Second, the tumor develops due to implantation of the appendiceal mucosa or dissemination of cancer cells during the initial appendectomy [22, 23]. The third type occurs when organs other than the appendix (ovaries, duplicated intestinal cysts, ileal diverticulum, etc.) are the primary sites [24–26]. In both cases presented here, there was no continuity between the tumor and the appendiceal root or cecum. In our first case, pathological examination confirmed the presence of a gastrointestinal wall structure with a muscular layer; therefore, the possibility of mucosal implantation seemed negative. Considering the surgical report that the appendix had been incompletely resected, although the root had been removed, and the fact that the mass on the CT image 1 month after the initial surgery closely resembled the appendiceal tip on the CT image immediately before the initial appendectomy, we concluded that it was extremely likely that the appendiceal tip was left behind and developed into a tumor. In our second case, the immunostaining results revealed a normal gastrointestinal wall structure; therefore, mucosal implantation did not appear. As 21 years have passed since the initial surgery, it is unlikely that the cancer cells were disseminated at the time of appendectomy. There has been no description suggesting the presence of an enteric duplication around the ileocecum in the initial surgical report. Furthermore, the non-neoplastic part of the epithelium covering the cystic cavity was not composed of small intestinal mucosa, which contains relatively few goblet cells, but of colonic mucosa, which consist of goblet cells almost entirely. This suggests that the cystic lesion was derived from the colon (including the appendix) rather than the ileum. Actually, true diverticulum with a muscular layer is not common in the terminal ileum, which is another underlying reason why we did not conceive the lesion was derived from ileal diverticulum. Based on these points, we concluded that the patient had undergone cecal resection as the initial surgery; however, the tip of the appendix was likely left behind.

Here, the question arises regarding the blood flow that allows the remnant appendix detached from the root to become a tumor. However, it has been suggested that the colonic mucosa is highly viable and can survive and continue to produce mucus, even when cut using a stapler and blood flow is lost [27]. In another case, Aida et al. reported that mucosal implantation grew on the abdominal wall and became a tumor [23]. In addition, Johnson et al. reported a case in which the appendiceal tip was left with a clear mesentery [6], and it is possible that the mesoappendix was also left in our case. Furthermore, it was possible that the remnant tip appendix received blood supply by adhesion to nearby organs, as reported by Parthsarathi et al. and Boardman et al. [8, 9].

In terms of treatment, regardless of whether the tumor is derived from the tip or the stump, the indications for surgery for appendiceal mucinous lesions remain the same. The tumor in our first case was an LAMN which developed 12 years after the initial surgery, and the tumor in our second case was a cancer that had ruptured 21 years after the initial surgery. Surgical removal of cystic lesions is essential before they rupture or develop into cancer. In addition, gentle intraoperative manipulation is required because rupture of mucinous lesions can lead to PMP. Recently, several cases of laparoscopic resection have been reported [8, 28]. As demonstrated in this case, the strategy of performing laparoscopic adhesiolysis and ileocecal mobilization, followed by open observation and resection of the tumor, is considered effective. Lymph node metastasis is rare [29] in cases of LAMN, and lymph node dissection is therefore generally considered unnecessary. In cases of malignancy, lymph node dissection is considered, but it has also been reported that lymph node metastasis is rarely observed in patients with well-differentiated mucinous adenocarcinoma and a Tis or T1 depth of invasion (Union for International Cancer Control 8th edition) [30]. However, it is challenging to differentiate between benign and malignant lesions using preoperative CT examinations [31], and CEA levels are believed to increase in approximately 30% of benign lesions [32, 33]. Therefore, it is not always easy to distinguish the two preoperatively.

In cases of rupture or mucinous dissemination, whether they are diagnosed as LAMN or mucinous adenocarcinoma, cytoreductive surgery plus hyperthermic intraperitoneal chemotherapy (CRS + HIPEC) should be considered [31, 32]. However, only a few facilities in Japan perform this procedure. In non-specialized facilities, the cytology of mucinous components, thorough irrigation of the abdominal cavity, and surgical incision with lavage are essential to minimize the implantation of tumor cells [33]. In our second case, extensive irrigation with a large volume of normal saline was performed intraoperatively, and there has been no recurrence for 32 months postoperatively, despite the absence of postoperative chemotherapy. PMP prognosis is believed to be associated with the presence of cellular components within the mucin and extent of mucinous seeding [31]. In our case, no distinct cellular components were detected within the mucin, and mucinous dissemination was limited to the region around the appendix, suggesting the possibility of a favorable outcome without recurrence. Continued follow-up of tumor markers and CT examinations are necessary in the future.

Fundamentally, when treating such patients, it is crucial to ensure that there is no residual disease during the initial surgery. Therefore, for cases of appendicitis complicated by periappendiceal abscesses, strategies such as interval appendectomy after initial treatments including intravenous antibiotic administration or percutaneous drainage may be effective [34, 35]. However, in cases of initial treatment failure or where inflammation remains strong, surgical intervention may be necessary. Especially in cases of severe inflammation to the extent that the whole appendix cannot be identified, clinicians should bear in mind that even after detachment at the base, remnant of the distal end may persist. If surgery is performed incompletely, appropriate follow-up is necessary. For example, by conducting a CT follow-up within a few months postoperatively, and if it confirms no residuals, we can consider concluding the follow-up. However, if residual tumors are detected, resection is basically recommended. When opting for observational management, it is recommended to remove the lesions if there are symptoms, tendencies to enlarge, or findings suggestive of neoplastic mucinous lesions. Although it is challenging to definitively distinguish between non-neoplastic and neoplastic lesions by radiologic study such as CT scan, features suggesting neoplasms such as wall calcifications, wall irregularity, diameter greater than 2cm, and absence of periappendiceal fat stranding were reported [36–38]. In any case, when observational management is chosen, follow-up may be long-term, and it is desirable to adopt an early resection strategy before rupturing or malignant transformation.

Conclusions

Herein, we report two cases of surgery for appendiceal neoplasms derived from appendiceal tip remnants after appendectomy. These experiences suggest that in cases where precise identification of the appendix is challenging because of severe inflammation during appendectomy, postoperative confirmation of whether an appendiceal remnant exists and appropriate follow-up are deemed necessary, considering the possibility that the appendiceal tip can remain intact even after detachment of the root side.