# Effects of polyphyllin Ⅶ on proliferation, apoptosis and cell cycle of diffuse large B-cell lymphoma cells

## TL;DR

This study shows that polyphyllin Ⅶ (PPⅦ) inhibits the growth of diffuse large B-cell lymphoma cells by promoting apoptosis and blocking the cell cycle.

## Contribution

The study demonstrates for the first time that PPⅦ induces apoptosis and cell cycle arrest in DLBCL cell lines.

## Key findings

- PPⅦ significantly suppressed the proliferation of U2932 and SUDHL-4 cells.
- PPⅦ increased apoptosis rates and upregulated apoptosis-related proteins while downregulating Bcl-2.
- PPⅦ induced G0/G1 phase arrest and reduced G2/M phase cells, with decreased expression of Cyclin D1, CDK4, CDK6, and Survivin.

## Abstract

探究重楼皂苷Ⅶ（PPⅦ）对弥漫大B细胞淋巴瘤（DLBCL）细胞株U2932和SUDHL-4的增殖、凋亡和细胞周期的影响。实验将DLBCL细胞株分为对照组和PPⅦ组，并使用MTT法、流式细胞术和Western blot法进行实验。结果显示，与对照组相比，PPⅦ显著抑制了U2932和SUDHL-4细胞的增殖（P<0.05）。细胞凋亡实验表明，0.5和1 µmol/L的PPⅦ处理使得两种细胞的凋亡率显著增加（P<0.05），并且凋亡相关蛋白的表达上调，而Bcl-2蛋白水平显著降低（P<0.05）。细胞周期实验显示，PPⅦ处理使得G0/G1期细胞增加（P<0.05），G2/M期细胞减少（P<0.05），且Cyclin D1、CDK4、CDK6和Survivin蛋白的表达量明显下调（P<0.05）。综上所述，PPⅦ通过抑制DLBCL细胞的增殖、促进细胞凋亡以及阻滞细胞于G0/G1期，发挥了抗淋巴瘤的作用。