Quantification of 11 metabolites in rat urine after exposure to organophosphates

Michael A. Leninsky; Vladislav E. Sobolev; Margarita O. Sokolova; Natalya G. Voitenko; Nikita V. Skvortsov

PMC · DOI:10.1186/s42826-024-00209-3·June 6, 2024

Quantification of 11 metabolites in rat urine after exposure to organophosphates

Michael A. Leninsky, Vladislav E. Sobolev, Margarita O. Sokolova, Natalya G. Voitenko, Nikita V. Skvortsov

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TL;DR

This study developed a method to measure 11 metabolites in rat urine to identify biomarkers for organophosphate poisoning.

Contribution

A new HPLC–MS/MS technique was developed for quantifying urine metabolites in organophosphate intoxication models.

Findings

01

The concentration of 11 metabolites changed similarly in rat urine after different organophosphate exposures.

02

The method reliably detected metabolite changes within 1–3 days post-exposure.

03

Adenine was the only metabolite that did not show consistent changes across all poisoning models.

Abstract

The aim of the study was to develop a technique for quantitative determination of rat urine metabolites by HPLC–MS/MS, which can be used to search for biomarkers of acute intoxication with organophosphates (OPs). The content of metabolites in the urine of rats exposed to a single dose of paraoxon (POX1x); interval, twice daily administration of paraoxon (POX2x); exposure to 2-(o-cresyl)-4H-1, 3, 2-benzodioxaphosphorin-2-oxide and paraoxon (CBPOX) was investigated. New data were obtained on the content in the urine of intact rats as well as rats in 3 models of OP poisoning: 3-methylhistidine, threonine, creatine, creatinine, lactic acid, acetylcarnitine, inosine, hypoxanthine, adenine, 3-hydroxymethyl-butyrate and 2-hydroxymethyl-butyrate. The proposed assay procedure is a simple and reliable tool for urine metabolomic studies. Within 1–3 days after OP exposure in all three models of…

Linked entities

Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.

Species1

Rattus norvegicus

Chemicals13

paraoxon

2-(o-cresyl)-4H-1, 3, 2-benzodioxaphosphorin-2-oxide

threonine acetylcarnitine creatinine OP 3-methylhistidine adenine inosine creatine hypoxanthine lactic acid 2-hydroxymethyl-butyrate

Diseases1

poisoning

Figures5

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Taxonomy

TopicsPesticide Exposure and Toxicity · Metabolomics and Mass Spectrometry Studies · Vitamin C and Antioxidants Research