# Ethanolic extract from Sophora moorcroftiana inhibit cell proliferation and alter the mechanical properties of human cervical cancer

## TL;DR

This study shows that an extract from Sophora moorcroftiana inhibits cervical cancer cell growth and changes their mechanical properties, suggesting potential as a low-toxicity treatment.

## Contribution

The study demonstrates the antiproliferative effects and biomechanical changes in cervical cancer cells caused by Sophora moorcroftiana extract.

## Key findings

- EESMS inhibited HeLa cell proliferation by blocking the G0/G1 phase and increasing Caspase-3 expression.
- EESMS altered the mechanical properties of HeLa cells at the single-cell level.
- 218 differentially expressed proteins were identified, involved in various cellular processes.

## Abstract

Cervical cancer is one of the most common gynecological malignancies. Previous studies have shown that the ethanol extract of Sophora moorcroftiana seeds (EESMS) possesses an antiproliferative effect on several tumors in vitro. Therefore, in this study, we assessed the impact of EESMS on human cervical carcinoma (HeLa) cell proliferation.

The proliferation and apoptotic effects of HeLa cells treated with EESMS were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, dual acridine orange/ethidium bromide double staining, flow cytometry, and western blotting. Single-cell level atomic force microscopy (AFM) was conducted to detect the mechanical properties of HeLa cells, and proteomics and bioinformatics methods were used to elucidate the molecular mechanisms of EESMS.

EESMS treatment inhibited HeLa cell proliferation by blocking the G0/G1 phase, increasing the expression of Caspase-3 and affecting its mechanical properties, and the EESMS indicated no significant inhibitory effect on mouse fibroblasts L929 cell line. In total, 218 differentially expressed proteins were identified using two-dimensional electrophoresis, and eight differentially expressed proteins were successfully identified using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. The differentially expressed proteins were involved in various cellular and biological processes.

This study provides a perspective on how cells change through biomechanics and a further theoretical foundation for the future application of Sophora moorcroftiana as a novel low-toxicity chemotherapy medication for treating human cervical cancer.

The online version contains supplementary material available at 10.1186/s12906-024-04502-5.