# TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections

## TL;DR

This paper introduces TSWIFT, a new method to repeatedly stain and analyze preserved human brain tissue, enabling detailed protein network studies.

## Contribution

The novel TSWIFT method allows iterative staining of pre-mounted FFPE brain sections for high-dimensional phenotyping.

## Key findings

- TSWIFT preserves tissue architecture and allows relabeling of a single FFPE sample over 10 times.
- The method enables high-dimensional analysis of cellular proteomes in mounted human brain tissue.
- TSWIFT is effective even after prolonged storage of samples at 4°C.

## Abstract

Comprehensive characterization of protein networks in mounted brain tissue represents a major challenge in brain and neurodegenerative disease research. In this study, we develop a simple staining method, called TSWIFT, to iteratively stain pre-mounted formalin fixed, paraffin embedded (FFPE) brain sections, thus enabling high-dimensional sample phenotyping. We show that TSWIFT conserves tissue architecture and allows for relabeling a single mounted FFPE sample more than 10 times, even after prolonged storage at 4 °C. Our results establish TSWIFT as an efficient method to obtain integrated high-dimensional knowledge of cellular proteomes by analyzing mounted FFPE human brain tissue.