# Cytotoxicity  of dental cement on soft tissue associated with dental implants at different time intervals

## TL;DR

This study examines how four types of dental cement affect human gum cells at different time intervals, finding varying levels of cell toxicity.

## Contribution

The study compares the cytotoxic effects of four dental cements on human gingival fibroblasts at multiple time points.

## Key findings

- Zinc oxide non-eugenol (ZOE) showed the highest cytotoxicity to human gingival fibroblasts.
- Cell viability was significantly reduced after exposure to all tested dental cements.
- RelyX U200 (RU200) exhibited the lowest cytotoxicity among the tested materials.

## Abstract

Background: To investigate and compare the effect of four commercially used dental cement at 24 hours, 48 hours,72 hours (h) and 6 days on the cellular response of human gingival fibroblast (HGF).

Methods: 3 cement pellet samples were made for each 4-test cement (n=12). The cement used for this study were zinc phosphate (ZP), zinc oxide non-eugenol (ZOE), RelyX U200 (RU200), and glass ionomer cement (GIC). The cytotoxicity of peri-implant tissues was investigated using one commercial cell line. All processing was done following International Organization for Standardization (ISO) methods 10993-5 and 10993-12 (MTT assay Test). Cell cultures without dental cement were considered as control. Standard laboratory procedures were followed to permit cell growth and confluence over 48 hrs after sub-cultivation. Before being subjected to analysis, the cells were kept in direct contact with the cement samples for the suggested time period. To validate the results the specimens were tested three times each. Cell death and inhibition of cell growth were measured quantitatively. Results were analyzed using 1-way ANOVA (a=0.05) followed by Tukey B post hoc test.

Results: The study showed the dental cement test material was cytotoxic. ZOE, ZP, GIC, and RU200 were cytotoxic in decreasing order, respectively, significantly reducing cell viability after exposure to HGF (p <0.001).

Conclusions: Within the limitations of this in-vitro cellular study, results indicated that HGF were vulnerable to the test the dental cement. The highest cytotoxicity was observed in ZOE, followed by ZP, GIC, and RU200.