# Enzyme-linked immunosorbent assay and immunohistochemical analysis of mast cell related biochemicals in oral submucous fibrosis

## TL;DR

This study investigates the role of mast cell-related chemicals in oral submucous fibrosis, a potentially malignant disorder, using ELISA and immunohistochemistry.

## Contribution

The study introduces a novel approach to analyze mast cell biochemicals in OSMF using both serum and tissue samples.

## Key findings

- Chymase, histamine, and diamine oxidase were detected in both serum and tissue samples.
- The study suggests a possible association between mast cell biochemicals and OSMF pathophysiology.
- Immunohistochemical analysis confirmed mast cell infiltration in submucosal tissues.

## Abstract

Oral submucous fibrosis (OSMF), a potentially malignant disorder, is developed by progressive fibrous tissue deposition in connective tissue along with atrophy of oral mucosa. Histological sections also show the mast cell infiltration in submucosa which may indicate their possible role in this entity. Abundant availability of biochemicals in mast cells like histamine and serine proteases like chymase may be released and play specific pathways in the disease pathophysiology. Possibly, if the histamine release has some part to play, diamine oxidase may also be found to have a relationship as it metabolizes histamine. The present study is proposed to identify the presence of chymase, histamine, and diamine oxidase in both, serum as well as tissue by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) respectively. This study may provide probable insight into the mast cell-related chemicals and their association with OSMF.