# The C. elegans SET1 histone methyltransferase SET-2 is not required for transgenerational memory of silencing

## TL;DR

This study investigates whether the SET-2 protein is needed for RNAi-induced gene silencing in C. elegans, finding it is not required for transgenerational silencing memory.

## Contribution

The study clarifies that RNAi pathways are not responsible for transcriptional misregulation in the absence of SET-2 in C. elegans.

## Key findings

- SET-2 is not required for RNAi-induced transgenerational silencing in C. elegans.
- RNAi pathway defects are not responsible for transcriptional misregulation in SET-2 absence.
- Endogenous small RNA pools remain unaffected without SET-2.

## Abstract

The
SET-2
/SET1 histone H3K4 methyltransferase and RNAi pathway components are required to maintain fertility across generations in
C. elegans
.
SET-2
preserves the germline transcriptional program transgenerationally, and RNAi pathways rely on small RNAs to establish and maintain transgenerational gene silencing. We investigated whether the functionality of RNAi-induced transgenerational silencing and the composition of pools of endogenous small RNA are affected by the absence of
SET-2
. Our results suggest that defects in RNAi pathways are not responsible for the transcriptional misregulation observed in the absence of
SET-2
.