# Unravelling epigenetic mechanisms in Cerastoderma edule genome: a comparison of healthy and neoplastic cockles

## TL;DR

This study compares the epigenetic profiles of healthy and cancerous cockles to understand how DNA methylation and hydroxymethylation contribute to transmissible bivalve neoplasia.

## Contribution

The study provides novel insights into the epigenetic mechanisms underlying transmissible neoplasia in cockles using whole-genome methylation and hydroxymethylation profiles.

## Key findings

- Neoplastic cockles show global hypomethylation compared to healthy ones.
- Hydroxymethylation levels are higher in neoplastic samples, especially in satellites and complex repeats.
- Retrotransposons exhibit distinct methylation and activity patterns in neoplastic cockles.

## Abstract

Cancer is a multifaceted genetic disease characterized by the acquisition of several essential hallmarks. Notably, certain cancers exhibit horizontal transmissibility, observed across mammalian species and diverse bivalves, the latter referred to as hemic neoplasia. Within this complex landscape, epigenetic mechanisms such as histone modifications and cytosine methylation emerge as fundamental contributors to the pathogenesis of these transmissible cancers. Our study delves into the epigenetic landscape of Cerastoderma edule, focusing on whole-genome methylation and hydroxymethylation profiles in heathy specimens and transmissible neoplasias by means of Nanopore long-read sequencing. Our results unveiled a global hypomethylation in the neoplastic specimens compared to their healthy counterparts, emphasizing the role of DNA methylation in these tumorigenic processes. Furthermore, we verified that intragenic CpG methylation positively correlated with gene expression, emphasizing its role in modulating transcription in healthy and neoplastic cockles, as also highlighted by some up-methylated oncogenic genes. Hydroxymethylation levels were significantly more elevated in the neoplastic samples, particularly within satellites and complex repeats, likely related to structural functions. Additionally, our analysis also revealed distinct methylation and activity patterns in retrotransposons, providing additional insights into bivalve neoplastic processes. Altogether, these findings contribute to understanding the epigenetic dynamics of bivalve neoplasias and shed light on the roles of DNA methylation and hydroxymethylation in tumorigenesis. Understanding these epigenetic alterations holds promise for advancing our broader understanding of cancer epigenetics.

The online version contains supplementary material available at 10.1007/s00438-024-02148-z.